Supramolecular organization of protein-releasing functional amyloids solved in bacterial inclusion bodies

Olivia Cano-Garrido, Escarlata Rodríguez-Carmona, César Díez-Gil, Esther Vázquez, Elisa Elizondo, Rafael Cubarsi, Joaquin Seras-Franzoso, José Luis Corchero, Ursula Rinas, Imma Ratera, Nora Ventosa, Jaume Veciana, Antonio Villaverde, Elena García-Fruitós

Research output: Contribution to journalArticleResearchpeer-review

46 Citations (Scopus)

Abstract

Slow protein release from amyloidal materials is a molecular platform used by nature to control protein hormone secretion in the endocrine system. The molecular mechanics of the sustained protein release from amyloids remains essentially unexplored. Inclusion bodies (IBs) are natural amyloids that occur as discrete protein nanoparticles in recombinant bacteria. These protein clusters have been recently explored as protein-based functional biomaterials with diverse biomedical applications, and adapted as nanopills to deliver recombinant protein drugs into mammalian cells. Interestingly, the slow protein release from IBs does not significantly affect the particulate organization and morphology of the material, suggesting the occurrence of a tight scaffold. Here, we have determined, by using a combined set of analytical approaches, a sponge-like supramolecular organization of IBs combining differently folded protein versions (amyloid and native-like), which supports both mechanical stability and sustained protein delivery. Apart from offering structural clues about how amyloid materials release their monomeric protein components, these findings open exciting possibilities for the tailored development of smart biofunctional materials, adapted to mimic the functions of amyloid-based secretory glands of higher organisms. © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)6134-6142
JournalActa Biomaterialia
Volume9
DOIs
Publication statusPublished - 1 Apr 2013

Keywords

  • Biomaterial
  • Functional amyloid
  • Nanomedicine
  • Nanoparticles
  • Protein release

Fingerprint Dive into the research topics of 'Supramolecular organization of protein-releasing functional amyloids solved in bacterial inclusion bodies'. Together they form a unique fingerprint.

Cite this