Suppression of phase and amplitude J(HH) modulations in HSQC experiments

Laura Castañar; Eduard Sistaré; Albert Virgili; R. Thomas Williamson; Teodor Parella

doi:10.1002/mrc.4149

Suppression of phase and amplitude J(HH) modulations in HSQC experiments

Laura Castañar, Eduard Sistaré, Albert Virgili, R. Thomas Williamson, Teodor Parella

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

35 Citations (Scopus)

Abstract

© 2014 John Wiley & Sons, Ltd. The amplitude and the phase of cross peaks in conventional 2D HSQC experiments are modulated by both proton-proton, J(HH), and proton-carbon, 1J(CH), coupling constants. It is shown by spectral simulation and experimentally that J(HH) interferences are suppressed in a novel perfect-HSQC pulse scheme that incorporates perfect-echo INEPT periods. The improved 2D spectra afford pure in-phase cross peaks with respect to 1J(CH) and J(HH), irrespective of the experiment delay optimization. In addition, peak volumes are not attenuated by the influence of J(HH), rendering practical issues such as phase correction, multiplet analysis, and signal integration more appropriate.

Original language	English
Pages (from-to)	115-119
Journal	Magnetic Resonance in Chemistry
Volume	53
Issue number	2
DOIs	https://doi.org/10.1002/mrc.4149
Publication status	Published - 5 Nov 2015

Keywords

HSQC
J(HH) modulation
NMR
Perfect HSQC
Pure in-phase signals

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10.1002/mrc.4149

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title = "Suppression of phase and amplitude J(HH) modulations in HSQC experiments",

abstract = "{\textcopyright} 2014 John Wiley & Sons, Ltd. The amplitude and the phase of cross peaks in conventional 2D HSQC experiments are modulated by both proton-proton, J(HH), and proton-carbon, 1J(CH), coupling constants. It is shown by spectral simulation and experimentally that J(HH) interferences are suppressed in a novel perfect-HSQC pulse scheme that incorporates perfect-echo INEPT periods. The improved 2D spectra afford pure in-phase cross peaks with respect to 1J(CH) and J(HH), irrespective of the experiment delay optimization. In addition, peak volumes are not attenuated by the influence of J(HH), rendering practical issues such as phase correction, multiplet analysis, and signal integration more appropriate.",

keywords = "HSQC, J(HH) modulation, NMR, Perfect HSQC, Pure in-phase signals",

author = "Laura Casta{\~n}ar and Eduard Sistar{\'e} and Albert Virgili and Williamson, {R. Thomas} and Teodor Parella",

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T1 - Suppression of phase and amplitude J(HH) modulations in HSQC experiments

AU - Castañar, Laura

AU - Sistaré, Eduard

AU - Virgili, Albert

AU - Williamson, R. Thomas

AU - Parella, Teodor

PY - 2015/11/5

Y1 - 2015/11/5

N2 - © 2014 John Wiley & Sons, Ltd. The amplitude and the phase of cross peaks in conventional 2D HSQC experiments are modulated by both proton-proton, J(HH), and proton-carbon, 1J(CH), coupling constants. It is shown by spectral simulation and experimentally that J(HH) interferences are suppressed in a novel perfect-HSQC pulse scheme that incorporates perfect-echo INEPT periods. The improved 2D spectra afford pure in-phase cross peaks with respect to 1J(CH) and J(HH), irrespective of the experiment delay optimization. In addition, peak volumes are not attenuated by the influence of J(HH), rendering practical issues such as phase correction, multiplet analysis, and signal integration more appropriate.

AB - © 2014 John Wiley & Sons, Ltd. The amplitude and the phase of cross peaks in conventional 2D HSQC experiments are modulated by both proton-proton, J(HH), and proton-carbon, 1J(CH), coupling constants. It is shown by spectral simulation and experimentally that J(HH) interferences are suppressed in a novel perfect-HSQC pulse scheme that incorporates perfect-echo INEPT periods. The improved 2D spectra afford pure in-phase cross peaks with respect to 1J(CH) and J(HH), irrespective of the experiment delay optimization. In addition, peak volumes are not attenuated by the influence of J(HH), rendering practical issues such as phase correction, multiplet analysis, and signal integration more appropriate.

KW - HSQC

KW - J(HH) modulation

KW - NMR

KW - Perfect HSQC

KW - Pure in-phase signals

U2 - 10.1002/mrc.4149

DO - 10.1002/mrc.4149

M3 - Article

VL - 53

SP - 115

EP - 119

IS - 2

ER -

Suppression of phase and amplitude J(HH) modulations in HSQC experiments

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Keywords

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