[3H]mepyramine binding to histamine H1 receptors in bovine retina

Lourdes Arbonés*, Enrique Claro, Fernando Picatoste, Agustina García

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

The promethazine-sensitive [3H]mepyramine binding was used to determine the presence of histamine H1 receptors in membranes from bovine retina. Specific mepyramine binding to retinal membranes was reversible, saturable and of high affinity. The apparent dissociation constant (KD=2.2±0.4 nM) and the density of binding sites (Bmax=60.9±5.1 fmol/mg protein), obtained in equilibrium studies, were similar to those found in bovine brain cortex. Binding was stereospecific and the inhibitory potencies of H1 and H2 antagonists indicated that [3H]mepyramine binding sites in the retina have characteristics of H1 receptors.

Original languageEnglish
Pages (from-to)445-450
Number of pages6
JournalBiochemical and biophysical research communications
Volume135
Issue number2
DOIs
Publication statusPublished - 13 Mar 1986

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