[3H]mepyramine binding to histamine H1 receptors in bovine retina

Lourdes Arbonés; Enrique Claro; Fernando Picatoste; Agustina García

doi:10.1016/0006-291X(86)90014-8

[³H]mepyramine binding to histamine H₁ receptors in bovine retina

Lourdes Arbonés^*, Enrique Claro, Fernando Picatoste, Agustina García

^*Corresponding author for this work

Department of Biochemistry and Molecular Biology

Universitat Autònoma de Barcelona (UAB)

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

The promethazine-sensitive [³H]mepyramine binding was used to determine the presence of histamine H₁ receptors in membranes from bovine retina. Specific mepyramine binding to retinal membranes was reversible, saturable and of high affinity. The apparent dissociation constant (K_D=2.2±0.4 nM) and the density of binding sites (B_max=60.9±5.1 fmol/mg protein), obtained in equilibrium studies, were similar to those found in bovine brain cortex. Binding was stereospecific and the inhibitory potencies of H₁ and H₂ antagonists indicated that [³H]mepyramine binding sites in the retina have characteristics of H₁ receptors.

Original language	English
Pages (from-to)	445-450
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	135
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(86)90014-8
Publication status	Published - 13 Mar 1986

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title = "[3H]mepyramine binding to histamine H1 receptors in bovine retina",

abstract = "The promethazine-sensitive [3H]mepyramine binding was used to determine the presence of histamine H1 receptors in membranes from bovine retina. Specific mepyramine binding to retinal membranes was reversible, saturable and of high affinity. The apparent dissociation constant (KD=2.2±0.4 nM) and the density of binding sites (Bmax=60.9±5.1 fmol/mg protein), obtained in equilibrium studies, were similar to those found in bovine brain cortex. Binding was stereospecific and the inhibitory potencies of H1 and H2 antagonists indicated that [3H]mepyramine binding sites in the retina have characteristics of H1 receptors.",

author = "Lourdes Arbon{\'e}s and Enrique Claro and Fernando Picatoste and Agustina Garc{\'i}a",

note = "Funding Information: This research was supported, in part, by a grant from the C.A.I.C.Y.T. Lourdes Arbor&s and Enrique Claro are recipients of fellowships from the {"}Plan de Formaci6n de Personal Investigador{"} of the Ministerio de Educacidn y Ciencia, Spain. We thank M.E. Palou and A. Burset for secretarial assistance. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "1986",

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doi = "10.1016/0006-291X(86)90014-8",

language = "English",

volume = "135",

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TY - JOUR

T1 - [3H]mepyramine binding to histamine H1 receptors in bovine retina

AU - Arbonés, Lourdes

AU - Claro, Enrique

AU - Picatoste, Fernando

AU - García, Agustina

N1 - Funding Information: This research was supported, in part, by a grant from the C.A.I.C.Y.T. Lourdes Arbor&s and Enrique Claro are recipients of fellowships from the "Plan de Formaci6n de Personal Investigador" of the Ministerio de Educacidn y Ciencia, Spain. We thank M.E. Palou and A. Burset for secretarial assistance. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 1986/3/13

Y1 - 1986/3/13

N2 - The promethazine-sensitive [3H]mepyramine binding was used to determine the presence of histamine H1 receptors in membranes from bovine retina. Specific mepyramine binding to retinal membranes was reversible, saturable and of high affinity. The apparent dissociation constant (KD=2.2±0.4 nM) and the density of binding sites (Bmax=60.9±5.1 fmol/mg protein), obtained in equilibrium studies, were similar to those found in bovine brain cortex. Binding was stereospecific and the inhibitory potencies of H1 and H2 antagonists indicated that [3H]mepyramine binding sites in the retina have characteristics of H1 receptors.

AB - The promethazine-sensitive [3H]mepyramine binding was used to determine the presence of histamine H1 receptors in membranes from bovine retina. Specific mepyramine binding to retinal membranes was reversible, saturable and of high affinity. The apparent dissociation constant (KD=2.2±0.4 nM) and the density of binding sites (Bmax=60.9±5.1 fmol/mg protein), obtained in equilibrium studies, were similar to those found in bovine brain cortex. Binding was stereospecific and the inhibitory potencies of H1 and H2 antagonists indicated that [3H]mepyramine binding sites in the retina have characteristics of H1 receptors.

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U2 - 10.1016/0006-291X(86)90014-8

DO - 10.1016/0006-291X(86)90014-8

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SN - 0006-291X

VL - 135

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EP - 450

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

[³H]mepyramine binding to histamine H₁ receptors in bovine retina

Abstract

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