Suitability of scoring PCC rings and fragments for dose assessment after high-dose exposures to ionizing radiation

Roser Puig, Leonardo Barrios, Mònica Pujol, Maria R.osa Caballín, Joan Francesc Barquinero

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

Copyright © 2013 Elsevier B.V. All rights reserved. Assessment of radiation doses through measurement of dicentric chromosomes may be difficult due to the inability of damaged cells to reach mitosis. After high-dose exposures, premature chromosome condensation (PCC) has become an important method in biodosimetry. PCC can be induced upon fusion with mitotic cells, or by treatment with chemicals such as calyculin A or okadaic acid. Several different cytogenetic endpoints have been measured with chemically induced PCC, e.g., via scoring of extra chromosome pieces or ring chromosomes. The dose-effect curves published with chemically induced PCC show differences in their coefficients and in the distribution of rings among cells. Here we present a study with calyculin A to induce PCC in peripheral blood lymphocytes irradiated at nine different doses of γ-rays up to 20Gy. Colcemid was also added in order to observe metaphase cells. During microscopical analysis the chromosome aberrations observed in the different cell-cycle phases (G2/M-PCC, M/A-PCC and M cells) were recorded. The proportion of G2/M-PCC cells was predominant from 3 to 20Gy, M cells decreased above 1Gy and M/A-PCC cells remained constant at all doses and showed the highest frequencies of PCC rings. Depending on the cell-cycle phase there was a difference in the linear coefficients in the dose-effect curves of extra fragments and rings. Poisson distribution among PCC rings was observed after calyculin A+colcemid treatment, facilitating the use of this methodology also for partial body exposures to high doses. This has been tested with two simulated partial exposures to 6 and 12Gy. The estimated doses in the irradiated fraction were very close to the real dose, indicating the usefulness of this methodology.
Original languageEnglish
Pages (from-to)1-7
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume757
DOIs
Publication statusPublished - 18 Sep 2013

Keywords

  • Biological dosimetry
  • Chromosome aberrations
  • High doses
  • Ionizing radiation

Fingerprint Dive into the research topics of 'Suitability of scoring PCC rings and fragments for dose assessment after high-dose exposures to ionizing radiation'. Together they form a unique fingerprint.

Cite this