Subtractive proteomic approach to the endometrial carcinoma invasion front

Marta Monge, Andreas Doll, Eva Colas, Antonio Gil-Moreno, Josep Castellvi, Angel Garcia, Nuria Colome, Asummpcio Perez-Benavente, Nuria Pedrola, Rafael Lopez-Lopez, Xavier Dolcet, Santiago Ramon Y Cajal, Jordi Xercavins, Xavier Matias-Guiu, Francesc Canals, Jaume Reventos, Miguel Abal

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19 Citations (Scopus)

Abstract

Tumor invasion defines the transition between tissue-restricted carcinomas, related to good outcome as optimal surgery becomes possible, and metastatic tumors associated with poor prognosis and a dramatic decrease in survival. In endometrial cancer, myometrial infiltration represents a determinant parameter highly valuable in prognosis. To date, the identification of proteins involved in endometrial carcinoma invasion has been essentially conducted by immunohistochemical methods, without a global perception on the invasive front. Laser microdissection presents nowadays limitations to the profound spatiotemporal regulation from both the tumor and the surrounding stroma occurring at the invasive front. In this work, we attempted an alternative proteomic approach to characterize specific components of the tumor invasive front or its reactive stroma, by comparing the invasive area of an endometrial carcinoma with the noninvasive superficial tumor area and normal tissue from the same patients. This strategy led us to identify proteins involved in cellular morphology, assembly and movement, differentially expressed at the invasive front, as well as pathways like cell-to-cell signaling and interaction and a modulated response to oxidative stress as events related to endometrial carcinoma invasion. In conclusion, we could identify new players of myometrial infiltration by applying a subtractive proteomic approach to the endometrial carcinoma invasion front. © 2009 American Chemical Society.
Original languageEnglish
Pages (from-to)4676-4684
JournalJournal of Proteome Research
Volume8
Issue number10
DOIs
Publication statusPublished - 19 Oct 2009

Keywords

  • 2D-DIGE
  • BLVRB
  • Endometrial carcinoma
  • Invasion front
  • Metastasis
  • SOD1
  • Subtractive proteomics

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  • Cite this

    Monge, M., Doll, A., Colas, E., Gil-Moreno, A., Castellvi, J., Garcia, A., Colome, N., Perez-Benavente, A., Pedrola, N., Lopez-Lopez, R., Dolcet, X., Ramon Y Cajal, S., Xercavins, J., Matias-Guiu, X., Canals, F., Reventos, J., & Abal, M. (2009). Subtractive proteomic approach to the endometrial carcinoma invasion front. Journal of Proteome Research, 8(10), 4676-4684. https://doi.org/10.1021/pr900390t