Substitution of transferrin by FeCl3 in the development of a low foetal calf serum concentration medium for KB-26.5 hybridoma cell line

Bo Damgaard, Anna Sanfeliu, Jordi Joan Cairó, Carles Casas, Carles Solà, Francesc Gòdia

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

KB-26.5, a murine hybridoma cell line producing an IgG3 monoclonal antibody used in blood type determination, primarily adapted to grow at 5% foetal calf serum (FCS) concentration has been adapted to grow at 0.5% FCS, maintaining its ability to produce antibodies at the same level. In the final step of adaptation, the addition of insulin, transferrin, ethanolamine and selenium to the media formulation was studied, using factorial assay techniques to check the effect of the different compounds and to optimize their required level for satisfactory growth and antibody secretion. KB-26.5 cells required only 20 μg/ml of transferrin to adapt to 0.5% FCS medium. Furthermore, transferrin could be substituted by FeCl3, at a relatively low level of 2 μg/ml. Maximum cell density decreased by 31.5% in spinner flask test, but the antibody titer was maintained, thus the specific productivity increased. However, inoculum size had to be increased three-fold with 0.5% FCS medium in order to assure cell growth. © 1993 Kluwer Academic Publishers.
Original languageEnglish
Pages (from-to)133-141
JournalCytotechnology
Volume13
Issue number2
DOIs
Publication statusPublished - 1 Jan 1993

Keywords

  • hybridoma cells
  • medium design
  • monoclonal antibody
  • transferrin substitution

Fingerprint Dive into the research topics of 'Substitution of transferrin by FeCl3 in the development of a low foetal calf serum concentration medium for KB-26.5 hybridoma cell line'. Together they form a unique fingerprint.

  • Cite this