Sub-chronic oral toxicity study in Sprague-Dawley rats with hypoxia mimetic cobalt chloride towards the development of promising neutraceutical for oxygen deprivation

Kalpana Shrivastava, Anju Bansal, Bhagwat Singh, Mustoori Sairam, Govindaswamy Ilavazhagan

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    Abstract

    The present study evaluates the toxicity from sub-chronic administration of CoCl2 (12.5mgcobaltkg-1 BW for 7 days) to male Sprague-Dawley rats in view of the beneficial effects of CoCl2 in animals and for developing efficacious therapeutic regimen in humans. 32 rats weighing 200±25g were used for all experiments. Blood was collected for hematological and biochemical analysis and various organs were dissected after perfusion of animals under anesthesia for other analyses. Mean feed consumption and feed conversion efficiency values were comparable across all study groups; however, hematological analysis depicted a significant increase in hemoglobin, hematocrit and RBC in the entire cobalt-supplemented groups, which are a component of its beneficial effect. There was a significant increase in monocytes, granulocytes and WBC after 1 and 24h, which were comparable with control after 7 days. Other biochemical analyses also showed no change with respect to control. Though the metal content increased significantly in liver initially (1 and 24h) after treatment, it was equivalent to control after 7 days. Moreover, histopathological analysis revealed no evidence of changes that could be attributed to cobalt pretreatment. It is therefore reasonable to conclude that the present study supports further use of the present dose of CoCl2, which was found to be nontoxic. © 2009 Elsevier GmbH.
    Original languageEnglish
    Pages (from-to)489-496
    JournalExperimental and Toxicologic Pathology
    Volume62
    Issue number5
    DOIs
    Publication statusPublished - 1 Sep 2010

    Keywords

    • CoCl 2
    • Hypoxia mimetic
    • Sprague-Dawley
    • Sub-chronic

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