Studying the T cell-astrocyte immune synapse

George P. Cribaro; Elena Saavedra-López; Paola V. Casanova; Laura Rodríguez; Carlos Barcia

doi:10.1007/978-1-4939-6881-7_32

Studying the T cell-astrocyte immune synapse

George P. Cribaro, Elena Saavedra-López, Paola V. Casanova, Laura Rodríguez, Carlos Barcia

Research output: Chapter in Book › Chapter › Research › peer-review

Abstract

© Springer Science+Business Media LLC 2017. In this chapter, we describe the technical details to visualize and analyze effector immunological synapses between T cells and astrocytes in the brain with high-resolution confocal imaging. This procedure is critical for the optimal and even penetration of labeling antibodies within the nerve tissue to obtain accurate staining and allow a uniform three-dimensional analysis of the T cell-astrocyte interactions. We emphasize here the comprehensive exploration of the tissue and analysis with confocal microscope as well as the display of microanatomical details of the three-dimensional reconstruction for interface visualization (including peripheral and central supramolecular activation clusters, effector molecules, and other organelles such as microtubule organizing centers (MTOCs) and Golgi apparatus).

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	517-531
Number of pages	14
Volume	1584
DOIs	https://doi.org/10.1007/978-1-4939-6881-7_32
Publication status	Published - 1 Jan 2017

Keywords

3D reconstruction
Astrocyte
Confocal microscopy
Immunofluorescence
T cell
Tissue
c-SMAC
p-SMAC

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10.1007/978-1-4939-6881-7_32

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title = "Studying the T cell-astrocyte immune synapse",

abstract = "{\textcopyright} Springer Science+Business Media LLC 2017. In this chapter, we describe the technical details to visualize and analyze effector immunological synapses between T cells and astrocytes in the brain with high-resolution confocal imaging. This procedure is critical for the optimal and even penetration of labeling antibodies within the nerve tissue to obtain accurate staining and allow a uniform three-dimensional analysis of the T cell-astrocyte interactions. We emphasize here the comprehensive exploration of the tissue and analysis with confocal microscope as well as the display of microanatomical details of the three-dimensional reconstruction for interface visualization (including peripheral and central supramolecular activation clusters, effector molecules, and other organelles such as microtubule organizing centers (MTOCs) and Golgi apparatus).",

keywords = "3D reconstruction, Astrocyte, Confocal microscopy, Immunofluorescence, T cell, Tissue, c-SMAC, p-SMAC",

author = "Cribaro, {George P.} and Elena Saavedra-L{\'o}pez and Casanova, {Paola V.} and Laura Rodr{\'i}guez and Carlos Barcia",

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doi = "10.1007/978-1-4939-6881-7_32",

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volume = "1584",

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T1 - Studying the T cell-astrocyte immune synapse

AU - Cribaro, George P.

AU - Saavedra-López, Elena

AU - Casanova, Paola V.

AU - Rodríguez, Laura

AU - Barcia, Carlos

PY - 2017/1/1

Y1 - 2017/1/1

N2 - © Springer Science+Business Media LLC 2017. In this chapter, we describe the technical details to visualize and analyze effector immunological synapses between T cells and astrocytes in the brain with high-resolution confocal imaging. This procedure is critical for the optimal and even penetration of labeling antibodies within the nerve tissue to obtain accurate staining and allow a uniform three-dimensional analysis of the T cell-astrocyte interactions. We emphasize here the comprehensive exploration of the tissue and analysis with confocal microscope as well as the display of microanatomical details of the three-dimensional reconstruction for interface visualization (including peripheral and central supramolecular activation clusters, effector molecules, and other organelles such as microtubule organizing centers (MTOCs) and Golgi apparatus).

AB - © Springer Science+Business Media LLC 2017. In this chapter, we describe the technical details to visualize and analyze effector immunological synapses between T cells and astrocytes in the brain with high-resolution confocal imaging. This procedure is critical for the optimal and even penetration of labeling antibodies within the nerve tissue to obtain accurate staining and allow a uniform three-dimensional analysis of the T cell-astrocyte interactions. We emphasize here the comprehensive exploration of the tissue and analysis with confocal microscope as well as the display of microanatomical details of the three-dimensional reconstruction for interface visualization (including peripheral and central supramolecular activation clusters, effector molecules, and other organelles such as microtubule organizing centers (MTOCs) and Golgi apparatus).

KW - 3D reconstruction

KW - Astrocyte

KW - Confocal microscopy

KW - Immunofluorescence

KW - T cell

KW - Tissue

KW - c-SMAC

KW - p-SMAC

U2 - 10.1007/978-1-4939-6881-7_32

DO - 10.1007/978-1-4939-6881-7_32

M3 - Chapter

SN - 1064-3745

VL - 1584

SP - 517

EP - 531

BT - Methods in Molecular Biology

ER -

Studying the T cell-astrocyte immune synapse

Abstract

Keywords

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