Studying the interactions of a platinum(ii) 9-aminoacridine complex with proteins and oligonucleotides by ESI-TOF MS

Katia G. Samper; Consuelo Vicente; Venancio Rodríguez; Sílvia Atrian; Natalia Cutillas; Mercè Capdevila; José Ruiz; Òscar Palacios

doi:10.1039/c1dt11506e

Studying the interactions of a platinum(ii) 9-aminoacridine complex with proteins and oligonucleotides by ESI-TOF MS

Katia G. Samper, Consuelo Vicente, Venancio Rodríguez, Sílvia Atrian, Natalia Cutillas, Mercè Capdevila, José Ruiz, Òscar Palacios

Department of Chemistry

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Abstract

The interaction of a novel Pt complex, [Pt(dmba)(N9-9AA)(PPh <inf>3</inf>)]<sup>+</sup>1 (dmba = N,N-dimethylbenzylamine-κN,κC; 9AA = 9-aminoacridine), which exhibits anti-tumor activity, with certain key proteins has been monitored by ESI-MS. Also, the interaction of 1 with a designed double-stranded oligonucleotide containing the GG motif has been followed by mass spectrometry as well as by fluorimetry. The results obtained show the low interaction of 1 with the considered proteins and the absence of covalent interaction with the oligonucleotides, but the fluorimetric data confirm the π-π interaction of 1 with the double-stranded DNA, which is probably the reason of the previously reported activity of 1 in several tumor cell lines. © The Royal Society of Chemistry.

Original language	English
Pages (from-to)	300-306
Journal	Dalton Transactions
Volume	41
Issue number	1
DOIs	https://doi.org/10.1039/c1dt11506e
Publication status	Published - 7 Jan 2012

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title = "Studying the interactions of a platinum(ii) 9-aminoacridine complex with proteins and oligonucleotides by ESI-TOF MS",

abstract = "The interaction of a novel Pt complex, [Pt(dmba)(N9-9AA)(PPh 3)]+1 (dmba = N,N-dimethylbenzylamine-κN,κC; 9AA = 9-aminoacridine), which exhibits anti-tumor activity, with certain key proteins has been monitored by ESI-MS. Also, the interaction of 1 with a designed double-stranded oligonucleotide containing the GG motif has been followed by mass spectrometry as well as by fluorimetry. The results obtained show the low interaction of 1 with the considered proteins and the absence of covalent interaction with the oligonucleotides, but the fluorimetric data confirm the π-π interaction of 1 with the double-stranded DNA, which is probably the reason of the previously reported activity of 1 in several tumor cell lines. {\textcopyright} The Royal Society of Chemistry.",

author = "Samper, {Katia G.} and Consuelo Vicente and Venancio Rodr{\'i}guez and S{\'i}lvia Atrian and Natalia Cutillas and Merc{\`e} Capdevila and Jos{\'e} Ruiz and {\`O}scar Palacios",

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T1 - Studying the interactions of a platinum(ii) 9-aminoacridine complex with proteins and oligonucleotides by ESI-TOF MS

AU - Samper, Katia G.

AU - Vicente, Consuelo

AU - Rodríguez, Venancio

AU - Atrian, Sílvia

AU - Cutillas, Natalia

AU - Capdevila, Mercè

AU - Ruiz, José

AU - Palacios, Òscar

PY - 2012/1/7

Y1 - 2012/1/7

N2 - The interaction of a novel Pt complex, [Pt(dmba)(N9-9AA)(PPh 3)]+1 (dmba = N,N-dimethylbenzylamine-κN,κC; 9AA = 9-aminoacridine), which exhibits anti-tumor activity, with certain key proteins has been monitored by ESI-MS. Also, the interaction of 1 with a designed double-stranded oligonucleotide containing the GG motif has been followed by mass spectrometry as well as by fluorimetry. The results obtained show the low interaction of 1 with the considered proteins and the absence of covalent interaction with the oligonucleotides, but the fluorimetric data confirm the π-π interaction of 1 with the double-stranded DNA, which is probably the reason of the previously reported activity of 1 in several tumor cell lines. © The Royal Society of Chemistry.

AB - The interaction of a novel Pt complex, [Pt(dmba)(N9-9AA)(PPh 3)]+1 (dmba = N,N-dimethylbenzylamine-κN,κC; 9AA = 9-aminoacridine), which exhibits anti-tumor activity, with certain key proteins has been monitored by ESI-MS. Also, the interaction of 1 with a designed double-stranded oligonucleotide containing the GG motif has been followed by mass spectrometry as well as by fluorimetry. The results obtained show the low interaction of 1 with the considered proteins and the absence of covalent interaction with the oligonucleotides, but the fluorimetric data confirm the π-π interaction of 1 with the double-stranded DNA, which is probably the reason of the previously reported activity of 1 in several tumor cell lines. © The Royal Society of Chemistry.

U2 - 10.1039/c1dt11506e

DO - 10.1039/c1dt11506e

M3 - Article

VL - 41

SP - 300

EP - 306

IS - 1

ER -

Studying the interactions of a platinum(ii) 9-aminoacridine complex with proteins and oligonucleotides by ESI-TOF MS

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