Study of miRNAs expression patterns in osteoporotic bone

Natalia García-Giralt, L. De-Ugarte, G. Yoskovitz, R. Güerri, D. Grinberg, X. Nogués, L. Mellibovsky, S. Balcells, S. Díez-Pérez

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Objectives: To identify microRNAs (miRNAs) differentially expressed in bone samples with osteoporotic fracture compared with healthy bones. Methods: Total RNA was extracted from fresh trabecular bone of the femoral neck of women undergoing hip replacement surgery, either because to osteoporotic fracture (n=6) or in the absence of osteoarthritis osteoporosis (based on BMD) (n=6). The samples were hybridized on an array of miRNAs and PCA diagrams and heat map were made. To compare expression levels, >1.5 times and a value p<0.05 Student's T test (corrected for multiple testing) was set as a threshold of significant change. Results: Both PCA analysis and the heat map showed a samples grouping whether there was fracture or not. 790 were detected miRNAs in bone samples, 82 of which were altered in the osteoporotic samples. After validation in another panel of 6 samples 6 osteoporotic and non-osteoporotic by PCR real time of the most significant miRNAs, and for which there was a test available, the miRNAs, miR-320a and miR- 22-3p were confirmed. These two miRNAs were detected in cultures of primary osteoblasts, although they did not maintain the same pattern of expression in total bone samples. Conclusions: We have shown that there are differences in the expression of miRNAs in samples with osteoporotic fracture. This opens prospects for research and design of new therapies.
Original languageEnglish
Pages (from-to)5-14
JournalRevista de Osteoporosis y Metabolismo Mineral
Volume8
Issue number1
Publication statusPublished - 1 Jan 2016

Keywords

  • Bone
  • Bone fracture
  • MicroRNA
  • Osteoblasts
  • Osteoporosis

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