Studies on Papain Catalyzed Synthesis of Gly-Phe in a Two-Liquid-Phase System

J. A. Feliu, C. De Mas, J. LóPez-SantíN

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3 Citations (Scopus)

Abstract

The interfacial stress has been identified as the major factor responsible for papain deactivation during the synthesis of Gly-Phe in a two-liquid-phase system. The enzyme has been stabilized by the addition of a surfactant (Tween 80) to the reaction mixture. The yield was thereby increased from 40% to as much as 70% under the given conditions. It has also been found that papain stability in a two-liquid-phase system is more closely related to interfacial tension and droplet size than to log P. © 1992 Elsevier B.V.
Original languageEnglish
Title of host publicationProgress in Biotechnology
Pages629-636
Number of pages7
Volume8
DOIs
Publication statusPublished - 1 Jan 1992

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