Structure of three Ribonuclease-A covalent derivatives with pyridoxal 5′-phosphate

Lluís Boqué; Maria G. Coll; Marc Ribó; Claudi M. Cuchillo; Ignacio Fita; Maria Vilanova

Structure of three Ribonuclease-A covalent derivatives with pyridoxal 5′-phosphate

Lluís Boqué, Maria G. Coll, Marc Ribó, Claudi M. Cuchillo, Ignacio Fita, Maria Vilanova

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Abstract

The crystal structure of three bovine pancreatic ribonuclease derivatives modified covalently with pyridoxal 5-phosphate at Lys-1 (derivative A), Lys-7 (derivative B) and Lys-41 (derivative C) has been determined. The structures found provide an explanation of the kinetic parameters obtained for derivatives A and B. In addition, these structures permit to postulate the contribution of Lys-7 and Lys-66 to the catalytic mechanism of ribonuclease A.

Original language	English
Pages (from-to)	101-108
Journal	Protein and Peptide Letters
Volume	5
Issue number	2
Publication status	Published - 1 Dec 1998

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@article{074df75580ba40b188e9c7410d8e2425,

title = "Structure of three Ribonuclease-A covalent derivatives with pyridoxal 5′-phosphate",

abstract = "The crystal structure of three bovine pancreatic ribonuclease derivatives modified covalently with pyridoxal 5-phosphate at Lys-1 (derivative A), Lys-7 (derivative B) and Lys-41 (derivative C) has been determined. The structures found provide an explanation of the kinetic parameters obtained for derivatives A and B. In addition, these structures permit to postulate the contribution of Lys-7 and Lys-66 to the catalytic mechanism of ribonuclease A.",

author = "Llu{\'i}s Boqu{\'e} and Coll, {Maria G.} and Marc Rib{\'o} and Cuchillo, {Claudi M.} and Ignacio Fita and Maria Vilanova",

year = "1998",

month = dec,

day = "1",

language = "English",

volume = "5",

pages = "101--108",

journal = "Protein and Peptide Letters",

issn = "0929-8665",

publisher = "Bentham Science Publishers B.V.",

number = "2",

}

TY - JOUR

T1 - Structure of three Ribonuclease-A covalent derivatives with pyridoxal 5′-phosphate

AU - Boqué, Lluís

AU - Coll, Maria G.

AU - Ribó, Marc

AU - Cuchillo, Claudi M.

AU - Fita, Ignacio

AU - Vilanova, Maria

PY - 1998/12/1

Y1 - 1998/12/1

N2 - The crystal structure of three bovine pancreatic ribonuclease derivatives modified covalently with pyridoxal 5-phosphate at Lys-1 (derivative A), Lys-7 (derivative B) and Lys-41 (derivative C) has been determined. The structures found provide an explanation of the kinetic parameters obtained for derivatives A and B. In addition, these structures permit to postulate the contribution of Lys-7 and Lys-66 to the catalytic mechanism of ribonuclease A.

AB - The crystal structure of three bovine pancreatic ribonuclease derivatives modified covalently with pyridoxal 5-phosphate at Lys-1 (derivative A), Lys-7 (derivative B) and Lys-41 (derivative C) has been determined. The structures found provide an explanation of the kinetic parameters obtained for derivatives A and B. In addition, these structures permit to postulate the contribution of Lys-7 and Lys-66 to the catalytic mechanism of ribonuclease A.

M3 - Article

VL - 5

SP - 101

EP - 108

JO - Protein and Peptide Letters

JF - Protein and Peptide Letters

SN - 0929-8665

IS - 2

ER -