The crystal structure of three bovine pancreatic ribonuclease derivatives modified covalently with pyridoxal 5-phosphate at Lys-1 (derivative A), Lys-7 (derivative B) and Lys-41 (derivative C) has been determined. The structures found provide an explanation of the kinetic parameters obtained for derivatives A and B. In addition, these structures permit to postulate the contribution of Lys-7 and Lys-66 to the catalytic mechanism of ribonuclease A.
|Journal||Protein and Peptide Letters|
|Publication status||Published - 1 Dec 1998|