Abstract
The crystal structure of three bovine pancreatic ribonuclease derivatives modified covalently with pyridoxal 5-phosphate at Lys-1 (derivative A), Lys-7 (derivative B) and Lys-41 (derivative C) has been determined. The structures found provide an explanation of the kinetic parameters obtained for derivatives A and B. In addition, these structures permit to postulate the contribution of Lys-7 and Lys-66 to the catalytic mechanism of ribonuclease A.
Original language | English |
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Pages (from-to) | 101-108 |
Journal | Protein and Peptide Letters |
Volume | 5 |
Issue number | 2 |
Publication status | Published - 1 Dec 1998 |