Structure-guided redesign of d-fructose-6-phosphate aldolase from E. coli: Remarkable activity and selectivity towards acceptor substrates by two-point mutation

Mariana Gutierrez, Teodor Parella, Jesús Joglar, Jordi Bujons, Pere Clapés

Research output: Contribution to journalArticleResearchpeer-review

33 Citations (Scopus)

Abstract

Structure-guided re-design of the acceptor binding site of d-fructose-6-phosphate aldolase from E. coli leads to the construction of FSA A129S/A165G double mutant with an activity between 5- to >900-fold higher than that of wild-type towards N-Cbz-aminoaldehyde derivatives. © 2011 The Royal Society of Chemistry.
Original languageEnglish
Pages (from-to)5762-5764
JournalChemical Communications
Volume47
Issue number20
DOIs
Publication statusPublished - 28 May 2011

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