Structure-based design of a B cell antigen from B. pseudomallei

Davide Gaudesi, Claudio Peri, Giacomo Quilici, Alessandro Gori, Mario Ferrer-Navarro, Oscar Conchillo-Solé, Rachael Thomas, Arnone Nithichanon, Ganjana Lertmemongkolchai, Richard Titball, Xavier Daura, Giorgio Colombo, Giovanna Musco

Research output: Contribution to journalArticleResearchpeer-review

12 Citations (Scopus)


© 2014 American Chemical Society. Burkholderia pseudomallei is the etiological agent of melioidosis, a severe endemic disease in South-East Asia, causing septicemia and organ failure with high mortality rates. Current treatments and diagnostic approaches are largely ineffective. The development of new diagnostic tools and vaccines toward effective therapeutic opportunities against B. pseudomallei is therefore an urgent priority. In the framework of a multidisciplinary project tackling melioidosis through reverse and structural vaccinology, BPSL1050 was identified as a candidate for immunodiagnostic and vaccine development based on its reactivity against the sera of melioidosis patients. We determined its NMR solution structure and dynamics, and by novel computational methods we predicted immunogenic epitopes that once synthesized were able to elicit the production of antibodies inducing the agglutination of the bacterium and recognizing both BPSL1050 and B. pseudomallei crude extracts. Overall, these results hold promise for novel chemical biology approaches in the discovery of new diagnostic and prophylactic tools against melioidosis.
Original languageEnglish
Pages (from-to)803-812
JournalACS Chemical Biology
Issue number3
Publication statusPublished - 20 Mar 2015


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