Structural characterization of the porcine pyruvate carboxylase (PC) gene

Guillermo Dávalos; Armand Sànchez; Oriol Vidal; José Luís Noguera; Marcel Amills

doi:10.1046/j.1439-0388.2003.00403.x

Structural characterization of the porcine pyruvate carboxylase (PC) gene

Guillermo Dávalos, Armand Sànchez, Oriol Vidal, José Luís Noguera, Marcel Amills

Department of Animal and Food Science

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

We have characterized the near complete coding sequence of the pig pyruvate carboxylase (PC) gene in six individuals belonging to the Iberian, Large White and Piétrain breeds. The pig PC nucleotide sequence was 3. 517 bp long and encoded one 1.171 amino acid protein displaying a remarkable level of amino acid identity (75-92%) with other vertebrate pyruvate carboxylase sequences. The pig PC mRNA was expressed in several tissues such as liver, backfat, abdominal fat, ovary, uterus and testicle. Sequence aligment allowed to identify one silent (C/T)837 polymorphism at exon 6 that was typed in a number of Piétrain, Landrace and Large White pigs by using a primer extension-based approach. Allelic frequencies were 0.23 (Piétrain), 0.64 (Landrace) and 0.47 (Large White) for the C allele. Radiation hybrid mapping of the pig PC locus revealed that this gene maps to 2p17, near the SW2623 and the SW256 genetic markers.

Original language	English
Pages (from-to)	338-345
Journal	Journal of Animal Breeding and Genetics
Volume	120
Issue number	5
DOIs	https://doi.org/10.1046/j.1439-0388.2003.00403.x
Publication status	Published - 1 Oct 2003

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title = "Structural characterization of the porcine pyruvate carboxylase (PC) gene",

abstract = "We have characterized the near complete coding sequence of the pig pyruvate carboxylase (PC) gene in six individuals belonging to the Iberian, Large White and Pi{\'e}train breeds. The pig PC nucleotide sequence was 3. 517 bp long and encoded one 1.171 amino acid protein displaying a remarkable level of amino acid identity (75-92%) with other vertebrate pyruvate carboxylase sequences. The pig PC mRNA was expressed in several tissues such as liver, backfat, abdominal fat, ovary, uterus and testicle. Sequence aligment allowed to identify one silent (C/T)837 polymorphism at exon 6 that was typed in a number of Pi{\'e}train, Landrace and Large White pigs by using a primer extension-based approach. Allelic frequencies were 0.23 (Pi{\'e}train), 0.64 (Landrace) and 0.47 (Large White) for the C allele. Radiation hybrid mapping of the pig PC locus revealed that this gene maps to 2p17, near the SW2623 and the SW256 genetic markers.",

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doi = "10.1046/j.1439-0388.2003.00403.x",

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T1 - Structural characterization of the porcine pyruvate carboxylase (PC) gene

AU - Dávalos, Guillermo

AU - Sànchez, Armand

AU - Vidal, Oriol

AU - Noguera, José Luís

AU - Amills, Marcel

PY - 2003/10/1

Y1 - 2003/10/1

N2 - We have characterized the near complete coding sequence of the pig pyruvate carboxylase (PC) gene in six individuals belonging to the Iberian, Large White and Piétrain breeds. The pig PC nucleotide sequence was 3. 517 bp long and encoded one 1.171 amino acid protein displaying a remarkable level of amino acid identity (75-92%) with other vertebrate pyruvate carboxylase sequences. The pig PC mRNA was expressed in several tissues such as liver, backfat, abdominal fat, ovary, uterus and testicle. Sequence aligment allowed to identify one silent (C/T)837 polymorphism at exon 6 that was typed in a number of Piétrain, Landrace and Large White pigs by using a primer extension-based approach. Allelic frequencies were 0.23 (Piétrain), 0.64 (Landrace) and 0.47 (Large White) for the C allele. Radiation hybrid mapping of the pig PC locus revealed that this gene maps to 2p17, near the SW2623 and the SW256 genetic markers.

AB - We have characterized the near complete coding sequence of the pig pyruvate carboxylase (PC) gene in six individuals belonging to the Iberian, Large White and Piétrain breeds. The pig PC nucleotide sequence was 3. 517 bp long and encoded one 1.171 amino acid protein displaying a remarkable level of amino acid identity (75-92%) with other vertebrate pyruvate carboxylase sequences. The pig PC mRNA was expressed in several tissues such as liver, backfat, abdominal fat, ovary, uterus and testicle. Sequence aligment allowed to identify one silent (C/T)837 polymorphism at exon 6 that was typed in a number of Piétrain, Landrace and Large White pigs by using a primer extension-based approach. Allelic frequencies were 0.23 (Piétrain), 0.64 (Landrace) and 0.47 (Large White) for the C allele. Radiation hybrid mapping of the pig PC locus revealed that this gene maps to 2p17, near the SW2623 and the SW256 genetic markers.

U2 - 10.1046/j.1439-0388.2003.00403.x

DO - 10.1046/j.1439-0388.2003.00403.x

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SP - 338

EP - 345

JO - Journal of Animal Breeding and Genetics

JF - Journal of Animal Breeding and Genetics

IS - 5

ER -

Structural characterization of the porcine pyruvate carboxylase (PC) gene

Abstract

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