Stat1 implication in the immune response to superantigens in vivo

Ramon Gimeno, Jordi Codony-Servat, Montserrat Plana, Jose Luis Rodriguez-Sanchez, Candido Juarez

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    Abstract

    We analyzed the activation and changes in the protein level of STAT1 as a consequence of in vivo treatment with superantigens. Ninety minutes after i.p. injection of the staphylococcal enterotoxin B (SEB), a complex containing STAT1 that was able to specifically bind to DNA containing GAS- like sequences was activated in mouse splenocytes. This complex had the same characteristics as that induced by IFN-γ in several in vitro systems. Activation of the complex was inhibited by cyclosporin A, and Abs against IFN-γ severely decreased the amount of complex detected. When splenocytes were analyzed 24 h after SEB treatment, a high increase in the amount of the STAT1 isoforms, STAT91 and STAT84, was observed by Western analysis, but binding to GAS-like sequences was clearly decreased when compared with analysis at 90 min. Nevertheless, when SEB was injected a second time 24 h after the first injection, the binding of STAT1 to GAS-like sequences had risen again. This approach corroborates the implication of IFN-γ in the response to superantigens in vivo and shows the relevance of analysis of transcription factors in defining the molecular events involved in the immune response.
    Original languageEnglish
    Pages (from-to)1378-1386
    JournalJournal of Immunology
    Volume156
    Issue number4
    Publication statusPublished - 15 Feb 1996

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