Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study

Darragh Duffy; Vincent Rouilly; Cecile Braudeau; Véronique Corbière; Raouf Djebali; Marie Noelle Ungeheuer; Regis Josien; Samuel T. LaBrie; Olivier Lantz; Delphine Louis; Eva Martinez-Caceres; Francoise Mascart; Jose G. Ruiz de Morales; Catherine Ottone; Lydia Redjah; Nina Salabert Le Guen; Alain Savenay; Manfred Schmolz; Antoine Toubert; Matthew L. Albert

doi:10.1016/j.clim.2017.09.019

Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study

Darragh Duffy, Vincent Rouilly, Cecile Braudeau, Véronique Corbière, Raouf Djebali, Marie Noelle Ungeheuer, Regis Josien, Samuel T. LaBrie, Olivier Lantz, Delphine Louis, Eva Martinez-Caceres, Francoise Mascart, Jose G. Ruiz de Morales, Catherine Ottone, Lydia Redjah, Nina Salabert Le Guen, Alain Savenay, Manfred Schmolz, Antoine Toubert, Matthew L. Albert

Department of Cellular Biology, Physiology and Immunology

Research output: Contribution to journal › Article › Research › peer-review

44 Citations (Scopus)

Abstract

© 2017 Functional immune responses are increasingly important for clinical studies, providing in depth biomarker information to assess immunotherapy or vaccination. Incorporating functional immune assays into routine clinical practice has remained limited due to challenges in standardizing sample preparation. We recently described the use of a whole blood syringe-based system, TruCulture® which permits point-of-care standardized immune stimulation. Here, we report on a multi-center clinical study in seven FOCIS Centers of Excellence to directly compare TruCulture to conventional PBMC methods. Whole blood and PBMCs from healthy donors were exposed to LPS, anti-CD3 anti-CD28 antibodies, or media alone. 55 protein analytes were analyzed centrally by Luminex multi-analyte profiling in a CLIA-certified laboratory. TruCulture responses showed greater reproducibility and improved the statistical power for monitoring differential immune response activation. The use of TruCulture addresses a major unmet need through a robust and flexible method for immunomonitoring that can be reproducibly applied in multi-center clinical studies. One sentence summary A multi-center study revealed greater reproducibility from whole blood stimulation systems as compared to PBMC stimulation for studying induced immune responses.

Original language	English
Pages (from-to)	325-335
Journal	Clinical Immunology
Volume	183
DOIs	https://doi.org/10.1016/j.clim.2017.09.019
Publication status	Published - 1 Oct 2017

Keywords

Cytokines
Functional immune responses
Immunomonitoring
Multi-center clinical studies
PBMCs
Whole blood

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.clim.2017.09.019

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Duffy, D., Rouilly, V., Braudeau, C., Corbière, V., Djebali, R., Ungeheuer, M. N., Josien, R., LaBrie, S. T., Lantz, O., Louis, D., Martinez-Caceres, E., Mascart, F., Ruiz de Morales, J. G., Ottone, C., Redjah, L., Guen, N. S. L., Savenay, A., Schmolz, M., Toubert, A., & Albert, M. L. (2017). Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study. Clinical Immunology, 183, 325-335. https://doi.org/10.1016/j.clim.2017.09.019

@article{c678a389a8f741c6aba032f1d92385af,

title = "Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study",

abstract = "{\textcopyright} 2017 Functional immune responses are increasingly important for clinical studies, providing in depth biomarker information to assess immunotherapy or vaccination. Incorporating functional immune assays into routine clinical practice has remained limited due to challenges in standardizing sample preparation. We recently described the use of a whole blood syringe-based system, TruCulture{\textregistered} which permits point-of-care standardized immune stimulation. Here, we report on a multi-center clinical study in seven FOCIS Centers of Excellence to directly compare TruCulture to conventional PBMC methods. Whole blood and PBMCs from healthy donors were exposed to LPS, anti-CD3 anti-CD28 antibodies, or media alone. 55 protein analytes were analyzed centrally by Luminex multi-analyte profiling in a CLIA-certified laboratory. TruCulture responses showed greater reproducibility and improved the statistical power for monitoring differential immune response activation. The use of TruCulture addresses a major unmet need through a robust and flexible method for immunomonitoring that can be reproducibly applied in multi-center clinical studies. One sentence summary A multi-center study revealed greater reproducibility from whole blood stimulation systems as compared to PBMC stimulation for studying induced immune responses.",

keywords = "Cytokines, Functional immune responses, Immunomonitoring, Multi-center clinical studies, PBMCs, Whole blood",

author = "Darragh Duffy and Vincent Rouilly and Cecile Braudeau and V{\'e}ronique Corbi{\`e}re and Raouf Djebali and Ungeheuer, {Marie Noelle} and Regis Josien and LaBrie, {Samuel T.} and Olivier Lantz and Delphine Louis and Eva Martinez-Caceres and Francoise Mascart and {Ruiz de Morales}, {Jose G.} and Catherine Ottone and Lydia Redjah and Guen, {Nina Salabert Le} and Alain Savenay and Manfred Schmolz and Antoine Toubert and Albert, {Matthew L.}",

year = "2017",

month = oct,

day = "1",

doi = "10.1016/j.clim.2017.09.019",

language = "English",

volume = "183",

pages = "325--335",

journal = "Clinical Immunology",

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publisher = "Academic Press Inc.",

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Duffy, D, Rouilly, V, Braudeau, C, Corbière, V, Djebali, R, Ungeheuer, MN, Josien, R, LaBrie, ST, Lantz, O, Louis, D, Martinez-Caceres, E, Mascart, F, Ruiz de Morales, JG, Ottone, C, Redjah, L, Guen, NSL, Savenay, A, Schmolz, M, Toubert, A & Albert, ML 2017, 'Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study', Clinical Immunology, vol. 183, pp. 325-335. https://doi.org/10.1016/j.clim.2017.09.019

TY - JOUR

T1 - Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study

AU - Duffy, Darragh

AU - Rouilly, Vincent

AU - Braudeau, Cecile

AU - Corbière, Véronique

AU - Djebali, Raouf

AU - Ungeheuer, Marie Noelle

AU - Josien, Regis

AU - LaBrie, Samuel T.

AU - Lantz, Olivier

AU - Louis, Delphine

AU - Martinez-Caceres, Eva

AU - Mascart, Francoise

AU - Ruiz de Morales, Jose G.

AU - Ottone, Catherine

AU - Redjah, Lydia

AU - Guen, Nina Salabert Le

AU - Savenay, Alain

AU - Schmolz, Manfred

AU - Toubert, Antoine

AU - Albert, Matthew L.

PY - 2017/10/1

Y1 - 2017/10/1

N2 - © 2017 Functional immune responses are increasingly important for clinical studies, providing in depth biomarker information to assess immunotherapy or vaccination. Incorporating functional immune assays into routine clinical practice has remained limited due to challenges in standardizing sample preparation. We recently described the use of a whole blood syringe-based system, TruCulture® which permits point-of-care standardized immune stimulation. Here, we report on a multi-center clinical study in seven FOCIS Centers of Excellence to directly compare TruCulture to conventional PBMC methods. Whole blood and PBMCs from healthy donors were exposed to LPS, anti-CD3 anti-CD28 antibodies, or media alone. 55 protein analytes were analyzed centrally by Luminex multi-analyte profiling in a CLIA-certified laboratory. TruCulture responses showed greater reproducibility and improved the statistical power for monitoring differential immune response activation. The use of TruCulture addresses a major unmet need through a robust and flexible method for immunomonitoring that can be reproducibly applied in multi-center clinical studies. One sentence summary A multi-center study revealed greater reproducibility from whole blood stimulation systems as compared to PBMC stimulation for studying induced immune responses.

AB - © 2017 Functional immune responses are increasingly important for clinical studies, providing in depth biomarker information to assess immunotherapy or vaccination. Incorporating functional immune assays into routine clinical practice has remained limited due to challenges in standardizing sample preparation. We recently described the use of a whole blood syringe-based system, TruCulture® which permits point-of-care standardized immune stimulation. Here, we report on a multi-center clinical study in seven FOCIS Centers of Excellence to directly compare TruCulture to conventional PBMC methods. Whole blood and PBMCs from healthy donors were exposed to LPS, anti-CD3 anti-CD28 antibodies, or media alone. 55 protein analytes were analyzed centrally by Luminex multi-analyte profiling in a CLIA-certified laboratory. TruCulture responses showed greater reproducibility and improved the statistical power for monitoring differential immune response activation. The use of TruCulture addresses a major unmet need through a robust and flexible method for immunomonitoring that can be reproducibly applied in multi-center clinical studies. One sentence summary A multi-center study revealed greater reproducibility from whole blood stimulation systems as compared to PBMC stimulation for studying induced immune responses.

KW - Cytokines

KW - Functional immune responses

KW - Immunomonitoring

KW - Multi-center clinical studies

KW - PBMCs

KW - Whole blood

U2 - 10.1016/j.clim.2017.09.019

DO - 10.1016/j.clim.2017.09.019

M3 - Article

SN - 1521-6616

VL - 183

SP - 325

EP - 335

JO - Clinical Immunology

JF - Clinical Immunology

ER -

Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study

Abstract

Keywords

UN SDGs

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