Stable HIV-1 integrase diversity during initial HIV-1 RNA Decay suggests complete blockade of plasma HIV-1 replication by effective raltegravir- containing salvage therapy

Marc Noguera-Julian, Maria Casadellà, Christian Pou, Cristina Rodríguez, Susana Pérez-Álvarez, Jordi Puig, Bonaventura Clotet, Roger Paredes

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

Background: There is legitimate concern that minority drug-resistant mutants may be selected during the initial HIV-1 RNA decay phase following antiretroviral therapy initiation, thus undermining efficacy of treatment. The goal of this study was to characterize viral resistance emergence and address viral population evolution during the first phase of viral decay after treatment containing initiation. Findings. 454 sequencing was used to characterize viral genetic diversity and polymorphism composition of the HIV-1 integrase gene during the first two weeks following initiation of raltegravir-containing HAART in four ART-experienced subjects. No low-prevalence Raltegravir (RAL) drug resistance mutations (DRM) were found at baseline. All patients undergoing treatment received a fully active ART according to GSS values (GSS ≥ 3.5). No emergence of DRM after treatment initiation was detected. Longitudinal analysis showed no evidence of any other polymorphic mutation emergence or variation in viral diversity indexes. Conclusions: This suggests that fully active salvage antiretroviral therapy including raltegravir achieves a complete blockade of HIV-1 replication in plasma. It is unlikely that raltegravir-resistant HIV-1 may be selected in plasma during the early HIV-1 RNA decay after treatment initiation if the administered therapy is active enough. © 2013 Noguera-Julian et al.; licensee BioMed Central Ltd.
Original languageEnglish
Article number350
JournalVirology Journal
Volume10
DOIs
Publication statusPublished - 5 Dec 2013

Keywords

  • Deep sequencing
  • Dynamics
  • HIV-1
  • Raltegravir

Fingerprint Dive into the research topics of 'Stable HIV-1 integrase diversity during initial HIV-1 RNA Decay suggests complete blockade of plasma HIV-1 replication by effective raltegravir- containing salvage therapy'. Together they form a unique fingerprint.

Cite this