Sperm characteristics and heterologous in vitro fertilisation capacity of Iberian ibex (Capra pyrenaica) epididymal sperm, frozen in the presence of the enzymatic antioxidant catalase

J. López-Saucedo, M. T. Paramio, R. Fierro, D. Izquierdo, M. G. Catalá, M. A. Coloma, A. Toledano-Díaz, A. López-Sebastián, J. Santiago-Moreno

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Abstract

The aim of this work was to evaluate the protective effect of catalase (CAT) on frozen/thawed ibex epididymal sperm recovered post mortem, and to detect any harmful effect this might have on sperm fertilisation capacity. Epididymal spermatozoa were diluted using a Tris-citric acid-glucose medium (TCG) composed of 3.8% Tris (w/v), 2.2% citric acid (w/v), 0.6% glucose (w/v), 5% glycerol (v/v), and 6% egg yolk (v/v). Sperm masses from the right epididymis were diluted with TCG medium, while those from the left were diluted with TCG medium supplemented with 200. IU/mL CAT. Heterologous in vitro fertilisation (IVF) was used to assess the fertilisation capacity of this sperm. The addition of CAT to the extender did not improve frozen/thawed sperm variables. Moreover, a reduced fertilisation capacity was detected: sperm diluted with TCG provided 25.5% 2PN zygotes, while just 13.2% was recorded for that diluted with TCG-CAT (P<. 0.01). The percentage of cleaved embryos at 48. hpi was higher (P<. 0.01) with the TCG sperm than with the TCG-CAT sperm (16.7% vs. 7.6%). The use of 200. IU/mL CAT as an additive cannot, therefore, be recommended for the preservation of ibex epididymal sperm. Other antioxidants should, however, be tested in both this and related wild mountain ungulates. © 2014 Elsevier Inc.
Original languageEnglish
Pages (from-to)389-394
JournalCryobiology
Volume68
DOIs
Publication statusPublished - 1 Jan 2014

Keywords

  • Catalase
  • Epididymal sperm
  • Frozen-thawed semen
  • Heterologous in vitro fertilisation
  • Ibex

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