Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: An update

Camila Peres Rubio; Josefa Hernández-Ruiz; Silvia Martinez-Subiela; Asta Tvarijonaviciute; José Joaquin Ceron

doi:10.1186/s12917-016-0792-7

Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: An update

Camila Peres Rubio, Josefa Hernández-Ruiz, Silvia Martinez-Subiela, Asta Tvarijonaviciute, José Joaquin Ceron

Department of Animal Medicine and Surgery

Research output: Contribution to journal › Article › Research › peer-review

75 Citations (Scopus)

Abstract

© 2016 The Author(s). The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total antioxidant capacity (TAC) is an analyte frequently used to assess the antioxidant status of biological samples and can evaluate the antioxidant response against the free radicals produced in a given disease. Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC) are different assays described to determine TAC of a sample. This review explains the basis of each assay and their application in the determination of TAC in dogs, and also provides selected information about reports in humans for comparative purposes. It is concluded that, ideally, various different assays integrated in a panel should be used for TAC evaluation, since depending on the assay performed TAC results can be markedly different.

Original language	English
Article number	166
Journal	BMC Veterinary Research
Volume	12
Issue number	1
DOIs	https://doi.org/10.1186/s12917-016-0792-7
Publication status	Published - 15 Aug 2016

Keywords

Antioxidant activity
Canine serum
Cupric
Ferric
Human serum
Oxidative stress
Trolox

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abstract = "{\textcopyright} 2016 The Author(s). The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total antioxidant capacity (TAC) is an analyte frequently used to assess the antioxidant status of biological samples and can evaluate the antioxidant response against the free radicals produced in a given disease. Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC) are different assays described to determine TAC of a sample. This review explains the basis of each assay and their application in the determination of TAC in dogs, and also provides selected information about reports in humans for comparative purposes. It is concluded that, ideally, various different assays integrated in a panel should be used for TAC evaluation, since depending on the assay performed TAC results can be markedly different.",

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AU - Ceron, José Joaquin

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