Spanish HIV-1-infected long-term nonprogressors of more than 15 years have an increased frequency of the CX3CR1 249I variant allele

Francesc Vidal*, Consuelo Viladés, Pere Domingo, Montserrat Broch, Enric Pedrol, David Dalmau, Hernando Knobel, Joaquim Peraire, Cristina Gutiérrez, Ma Antònia Sambeat, Àngels Fontanet, Elisabeth Deig, Mireia Cairó, Milagros Montero, Cristóbal Richart, Simon Mallal

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

24 Citations (Scopus)

Abstract

Background and Objectives: The influence of the polymorphisms of the CX3CR1 chemokine receptor gene on the natural history of HIV-1 infection is controversial. This study aimed to determine whether functionally active CX3CR1 genetic variants are associated with long-term nonprogressive infection of > 15 years in HIV-1-infected Spanish patients. Patients and Methods: Two single-nucleotide polymorphisms, V249I (G > A) and T280M (C > T), of the CX3CR1 gene were assessed in 271 Spaniards. These included 60 HIV-1-infected patients who were long-term nonprogressors (LTNPs) of > 15 years, 109 HIV-1-infected patients who were usual progressors (UPs), and 102 control subjects. The CCR5Δ32 was also assessed. Genotyping was performed using polymerase chain reaction and automatic sequencing analysis methods on white cell DNA. Genotype and allele frequencies were compared by the χ2 test and the Fisher exact test. Results: The frequencies of the 249I variant allele were 42% for LTNPs, 24.5% for UPs, and 35% for healthy controls; the differences between LTNPs and UPs were significant (odds ratio 0.46; 95% CI: 0.27 to 0.75; P = 0.0017). For 280M the distribution was 16% for LTNPs, 14% for UPs, and 17% for healthy controls (P = NS). The haplotype 249I280T was significantly more common in LTNPs than in UPs (P = 0.0007). These results persisted after excluding from the analysis the individuals carrying the CCR5Δ32. Conclusions: CX3CR1 249I variant allele is more frequent in Spanish HIV-1-infected LTNPs of > 15 years. This effect is independent of the presence of the CCR5Δ32 allele.

Original languageAmerican English
Pages (from-to)527-531
Number of pages5
JournalJournal of Acquired Immune Deficiency Syndromes
Volume40
Issue number5
DOIs
Publication statusPublished - Dec 2005

Keywords

  • AIDS
  • CCR5
  • Chemokine receptor
  • Chemokines
  • CX3CR1
  • HIV-1 infection
  • Long-term nonprogressors

Fingerprint Dive into the research topics of 'Spanish HIV-1-infected long-term nonprogressors of more than 15 years have an increased frequency of the CX3CR1 249I variant allele'. Together they form a unique fingerprint.

Cite this