Small Ruminants: Prepubertal Oocyte Donors

Dolors Izquierdo*, María Gracia Catalá, María Teresa Paramio

*Corresponding author for this work

Research output: Chapter in BookChapterResearchpeer-review

Abstract

© Springer Science+Business Media, LLC, part of Springer Nature 2019. In vitro embryo production using oocytes of prepubertal females is named JIVET (juvenile in vitro embryo technologies). The aim of the JIVET is to increase genetic gain by shortening the generation interval in breeding programs. In this chapter we describe the methodology currently used in our laboratory for producing in vitro embryos from prepubertal sheep (90–120 days old) and goats (30–45 days old). Briefly, we obtain cumulus–oocyte complexes (COCs) from slaughtered female ovaries. These COCs are in vitro matured in TCM199 with 10% (v/v) fetal bovine serum (FBS) at 38.5 °C in a humidified air atmosphere with 5% CO2 for 24 h. The next day we collect ejaculates from males of proven fertility and select the most motile spermatozoa by density gradient. Following coincubation with mature COCs, presumptive zygotes and embryos are cultured in SOF for 8 days at 38.5 °C, 90% N2, 5% CO2, 5% O2, and 100% humidity to obtain blastocysts.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages155-163
Number of pages9
Volume2006
ISBN (Electronic)1940-6029
DOIs
Publication statusPublished - 1 Jan 2019

Publication series

NameMethods in Molecular Biology
Volume2006

Keywords

  • Embryo
  • Goat
  • In vitro culture
  • In vitro fertilization
  • In vitro maturation
  • Juvenile in vitro embryo technology
  • Oocyte
  • Prepubertal
  • Sheep

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