TY - JOUR
T1 - Skeletal muscle interleukin-6 regulates metabolic factors in iWAT during HFD and exercise training
AU - Knudsen, JG
AU - Bertholdt, L
AU - Joensen, E
AU - Lassen, SB
AU - Hidalgo, J
AU - Pilegaard, H.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - © 2015 The Obesity Society. Objective To investigate the role of skeletal muscle (SkM) interleukin (IL)-6 in the regulation of adipose tissue metabolism. Methods Muscle-specific IL-6 knockout (IL-6 MKO) and IL-6loxP/loxP (Floxed) mice were subjected to standard rodent diet (Chow), high-fat diet (HFD), or HFD in combination with exercise training (HFD ExTr) for 16 weeks. Results Total fat mass increased (P < 0.05) in both genotypes with HFD. However, HFD IL-6 MKO mice had lower (P < 0.05) inguinal adipose tissue (iWAT) mass than HFD Floxed mice. Accordingly, iWAT glucose transporter 4 (GLUT4) protein content, 5′AMP activated protein kinase (AMPK)Thr172 phosphorylation, and fatty acid synthase (FAS) mRNA content were lower (P < 0.05) in IL-6 MKO than Floxed mice on Chow. In addition, iWAT AMPKThr172 and hormone-sensitive lipase (HSL)Ser565 phosphorylation as well as perilipin protein content was higher (P < 0.05) in HFD IL-6 MKO than HFD Floxed mice, and pyruvate dehydrogenase E1α (PDH-E1α) protein content was higher (P < 0.05) in HFD ExTr IL-6 MKO than HFD ExTr Floxed mice. Conclusions These findings indicate that SkM IL-6 affects iWAT mass through regulation of glucose uptake capacity as well as lipogenic and lipolytic factors.
AB - © 2015 The Obesity Society. Objective To investigate the role of skeletal muscle (SkM) interleukin (IL)-6 in the regulation of adipose tissue metabolism. Methods Muscle-specific IL-6 knockout (IL-6 MKO) and IL-6loxP/loxP (Floxed) mice were subjected to standard rodent diet (Chow), high-fat diet (HFD), or HFD in combination with exercise training (HFD ExTr) for 16 weeks. Results Total fat mass increased (P < 0.05) in both genotypes with HFD. However, HFD IL-6 MKO mice had lower (P < 0.05) inguinal adipose tissue (iWAT) mass than HFD Floxed mice. Accordingly, iWAT glucose transporter 4 (GLUT4) protein content, 5′AMP activated protein kinase (AMPK)Thr172 phosphorylation, and fatty acid synthase (FAS) mRNA content were lower (P < 0.05) in IL-6 MKO than Floxed mice on Chow. In addition, iWAT AMPKThr172 and hormone-sensitive lipase (HSL)Ser565 phosphorylation as well as perilipin protein content was higher (P < 0.05) in HFD IL-6 MKO than HFD Floxed mice, and pyruvate dehydrogenase E1α (PDH-E1α) protein content was higher (P < 0.05) in HFD ExTr IL-6 MKO than HFD ExTr Floxed mice. Conclusions These findings indicate that SkM IL-6 affects iWAT mass through regulation of glucose uptake capacity as well as lipogenic and lipolytic factors.
U2 - https://doi.org/10.1002/oby.21139
DO - https://doi.org/10.1002/oby.21139
M3 - Article
SN - 1930-7381
VL - 23
SP - 1616
EP - 1624
JO - Obesity
JF - Obesity
IS - 8
ER -
