Abstract
© 2017 The Author(s). A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.
Original language | English |
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Article number | 45 |
Journal | Genome Biology |
Volume | 18 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1 Mar 2017 |
Keywords
- Conservation
- Cryopreservation
- MARS-Seq
- PBMC
- PDOX
- Patient-derived orthotopic xenograft
- Peripheral blood mononuclear cells
- RNA sequencing
- Single-cell genomics
- Smart-seq2
- Transcriptomics