Single-cell transcriptome conservation in cryopreserved cells and tissues

Amy Guillaumet-Adkins, Gustavo Rodríguez-Esteban, Elisabetta Mereu, Maria Mendez-Lago, Diego A. Jaitin, Alberto Villanueva, August Vidal, Alex Martinez-Marti, Enriqueta Felip, Ana Vivancos, Hadas Keren-Shaul, Simon Heath, Marta Gut, Ido Amit, Ivo Gut, Holger Heyn

Research output: Contribution to journalArticleResearchpeer-review

60 Citations (Scopus)

Abstract

© 2017 The Author(s). A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.
Original languageEnglish
Article number45
JournalGenome Biology
Volume18
Issue number1
DOIs
Publication statusPublished - 1 Mar 2017

Keywords

  • Conservation
  • Cryopreservation
  • MARS-Seq
  • Patient-derived orthotopic xenograft
  • PBMC
  • PDOX
  • Peripheral blood mononuclear cells
  • RNA sequencing
  • Single-cell genomics
  • Smart-seq2
  • Transcriptomics

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