Simultaneous spectrophotometric determination of levodopa and benserazide in a pharmaceutical preparation by use of partial least-squares regression

M. Blanco, J. Coello, H. Iturriaga, S. Maspoch, N. Villegas

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15 Citations (Scopus)

Abstract

A method based on a partial least-squares calibration (PLS) for the simultaneous spectrophotometric determination of levodopa (I) and benserazide (II) in a commercially available pharmaceutical preparation is proposed. The calibration procedure was developped by using laboratory made mixtures of I and II, and optimized by selecting the most suitable wavelength range and spectral mode. The relative standard error of prediction (RSEP %) was 0.5 % and 0.9% with a limiting reproducibility (R) of 4 10-6 M and 1.5 10-6 M for levodopa and benserazide, respectively The proposed method is straightforward, expeditious and precise. Its results are consistent with those provided by HPLC for the two analytes.
Original languageEnglish
Pages (from-to)2701-2718
JournalAnalytical Letters
Volume33
Issue number13
DOIs
Publication statusPublished - 1 Jan 2000

Keywords

  • Benserazide
  • Levodopa
  • Partial least-squares calibration
  • Pharmaceutical analysis
  • Spectrophotometric determination

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