Simultaneous on line monitoring of intracellular β-galactosidase activity and biomass using flow injection analysis in Escherichia coli batch fermentations

F. Valero, F. J. Lafuente, C. Solà, A. Benito, M. Vidal, J. Cairó, A. Villaverde

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

A novel method to monitor on-line intracellular β-galactosidase activity and biomass simultaneously, using flow injection analysis (FIA), has been developed. The automatic ultrasonic cell disruption and FIA analysis allow the processing of 10 samples/hour with a wide and variable linear working range of β-galactosidase activity and biomass and a maximum relative standard deviation (RSD) of 1.5%. The system has been optimized by monitoring biomass and intracellular β-galactosidase activity in Escherichia coli batch fermentation. © 1992 Science & Technology Letters.
Original languageEnglish
Pages (from-to)213-218
JournalBiotechnology Techniques
Volume6
Issue number3
DOIs
Publication statusPublished - 1 May 1992

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