Abstract
A novel method to monitor on-line intracellular β-galactosidase activity and biomass simultaneously, using flow injection analysis (FIA), has been developed. The automatic ultrasonic cell disruption and FIA analysis allow the processing of 10 samples/hour with a wide and variable linear working range of β-galactosidase activity and biomass and a maximum relative standard deviation (RSD) of 1.5%. The system has been optimized by monitoring biomass and intracellular β-galactosidase activity in Escherichia coli batch fermentation. © 1992 Science & Technology Letters.
Original language | English |
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Pages (from-to) | 213-218 |
Journal | Biotechnology Techniques |
Volume | 6 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1 May 1992 |