Simultaneous on line monitoring of intracellular β-galactosidase activity and biomass using flow injection analysis in Escherichia coli batch fermentations

F. Valero; F. J. Lafuente; C. Solà; A. Benito; M. Vidal; J. Cairó; A. Villaverde

doi:10.1007/BF02439346

Simultaneous on line monitoring of intracellular β-galactosidase activity and biomass using flow injection analysis in Escherichia coli batch fermentations

F. Valero, F. J. Lafuente, C. Solà, A. Benito, M. Vidal, J. Cairó, A. Villaverde

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Abstract

A novel method to monitor on-line intracellular β-galactosidase activity and biomass simultaneously, using flow injection analysis (FIA), has been developed. The automatic ultrasonic cell disruption and FIA analysis allow the processing of 10 samples/hour with a wide and variable linear working range of β-galactosidase activity and biomass and a maximum relative standard deviation (RSD) of 1.5%. The system has been optimized by monitoring biomass and intracellular β-galactosidase activity in Escherichia coli batch fermentation. © 1992 Science & Technology Letters.

Original language	English
Pages (from-to)	213-218
Journal	Biotechnology Techniques
Volume	6
Issue number	3
DOIs	https://doi.org/10.1007/BF02439346
Publication status	Published - 1 May 1992

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title = "Simultaneous on line monitoring of intracellular β-galactosidase activity and biomass using flow injection analysis in Escherichia coli batch fermentations",

abstract = "A novel method to monitor on-line intracellular β-galactosidase activity and biomass simultaneously, using flow injection analysis (FIA), has been developed. The automatic ultrasonic cell disruption and FIA analysis allow the processing of 10 samples/hour with a wide and variable linear working range of β-galactosidase activity and biomass and a maximum relative standard deviation (RSD) of 1.5%. The system has been optimized by monitoring biomass and intracellular β-galactosidase activity in Escherichia coli batch fermentation. {\textcopyright} 1992 Science & Technology Letters.",

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T1 - Simultaneous on line monitoring of intracellular β-galactosidase activity and biomass using flow injection analysis in Escherichia coli batch fermentations

AU - Valero, F.

AU - Lafuente, F. J.

AU - Solà, C.

AU - Benito, A.

AU - Vidal, M.

AU - Cairó, J.

AU - Villaverde, A.

PY - 1992/5/1

Y1 - 1992/5/1

N2 - A novel method to monitor on-line intracellular β-galactosidase activity and biomass simultaneously, using flow injection analysis (FIA), has been developed. The automatic ultrasonic cell disruption and FIA analysis allow the processing of 10 samples/hour with a wide and variable linear working range of β-galactosidase activity and biomass and a maximum relative standard deviation (RSD) of 1.5%. The system has been optimized by monitoring biomass and intracellular β-galactosidase activity in Escherichia coli batch fermentation. © 1992 Science & Technology Letters.

AB - A novel method to monitor on-line intracellular β-galactosidase activity and biomass simultaneously, using flow injection analysis (FIA), has been developed. The automatic ultrasonic cell disruption and FIA analysis allow the processing of 10 samples/hour with a wide and variable linear working range of β-galactosidase activity and biomass and a maximum relative standard deviation (RSD) of 1.5%. The system has been optimized by monitoring biomass and intracellular β-galactosidase activity in Escherichia coli batch fermentation. © 1992 Science & Technology Letters.

U2 - 10.1007/BF02439346

DO - 10.1007/BF02439346

M3 - Article

VL - 6

SP - 213

EP - 218

IS - 3

ER -

Simultaneous on line monitoring of intracellular β-galactosidase activity and biomass using flow injection analysis in Escherichia coli batch fermentations

Abstract

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