Abstract
Chirality plays a fundamental role in nature, but its detection and quantification still face many limitations. To date, the enantiospecific analysis of mixtures necessarily requires prior separation of the individual components. The simultaneous enantiospecific detection of multiple chiral molecules in a mixture represents a major challenge, which would lead to a significantly better understanding of the underlying biological processes; for example, via enantiospecifically analysing metabolites in their native environment. Here, we report on the first in situ enantiospecific detection of a thirty-nine-component mixture. As a proof of concept, eighteen essential amino acids at physiological concentrations were simultaneously enantiospecifically detected using NMR spectroscopy and a chiral solvating agent. This work represents a first step towards the simultaneous multicomponent enantiospecific analysis of complex mixtures, a capability that will have substantial impact on metabolism studies, metabolic phenotyping, chemical reaction monitoring, and many other fields where complex mixtures containing chiral molecules require efficient characterisation.
Original language | English |
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Pages (from-to) | 23615-23619 |
Number of pages | 5 |
Journal | Angewandte Chemie - International Edition |
Volume | 59 |
Issue number | 52 |
DOIs | |
Publication status | Published - 21 Dec 2020 |
Keywords
- complex mixture
- mixture effect
- multicomponent
- NMR spectroscopy
- simultaneous enantiospecific analysis
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Dive into the research topics of 'Simultaneous Enantiospecific Detection of Multiple Compounds in Mixtures using NMR Spectroscopy'. Together they form a unique fingerprint.Student theses
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Advances in NMR spectroscopic methodology and applications: time-efficient methods, ultra long-range heteronuclear correlation experiments and enantiospecific analysis of complex mixtures
Motiram Corral, K. (Author), Parella Coll, T. (Director), Perez Trujillo, M. (Director), Nolis Fañanas, P. (Director) & Jaime Cardiel, C. (Tutor), 21 Oct 2021Student thesis: Doctoral thesis