TY - JOUR
T1 - Simultaneous Enantiospecific Detection of Multiple Compounds in Mixtures using NMR Spectroscopy
AU - Kuhn, Lars T.
AU - Motiram-Corral, Kumar
AU - Athersuch, Toby J.
AU - Parella, Teodor
AU - Pérez-Trujillo, Míriam
N1 - Funding Information:
Financial support provided by the Spanish Ministry of Economy and Competitiveness (project PGC2018-095808-B-I00) is gratefully acknowledged. We also thank the ?Servei de Resson?ncia Magn?tica Nuclear (SeRMN)? of the Universitat Aut?noma de Barcelona (UAB) for the generous allocation of spectrometer time.
Funding Information:
Financial support provided by the Spanish Ministry of Economy and Competitiveness (project PGC2018‐095808‐B‐I00) is gratefully acknowledged. We also thank the “Servei de Ressonància Magnètica Nuclear (SeRMN)” of the Universitat Autònoma de Barcelona (UAB) for the generous allocation of spectrometer time.
Publisher Copyright:
© 2020 Wiley-VCH GmbH
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2020/12/21
Y1 - 2020/12/21
N2 - Chirality plays a fundamental role in nature, but its detection and quantification still face many limitations. To date, the enantiospecific analysis of mixtures necessarily requires prior separation of the individual components. The simultaneous enantiospecific detection of multiple chiral molecules in a mixture represents a major challenge, which would lead to a significantly better understanding of the underlying biological processes; for example, via enantiospecifically analysing metabolites in their native environment. Here, we report on the first in situ enantiospecific detection of a thirty-nine-component mixture. As a proof of concept, eighteen essential amino acids at physiological concentrations were simultaneously enantiospecifically detected using NMR spectroscopy and a chiral solvating agent. This work represents a first step towards the simultaneous multicomponent enantiospecific analysis of complex mixtures, a capability that will have substantial impact on metabolism studies, metabolic phenotyping, chemical reaction monitoring, and many other fields where complex mixtures containing chiral molecules require efficient characterisation.
AB - Chirality plays a fundamental role in nature, but its detection and quantification still face many limitations. To date, the enantiospecific analysis of mixtures necessarily requires prior separation of the individual components. The simultaneous enantiospecific detection of multiple chiral molecules in a mixture represents a major challenge, which would lead to a significantly better understanding of the underlying biological processes; for example, via enantiospecifically analysing metabolites in their native environment. Here, we report on the first in situ enantiospecific detection of a thirty-nine-component mixture. As a proof of concept, eighteen essential amino acids at physiological concentrations were simultaneously enantiospecifically detected using NMR spectroscopy and a chiral solvating agent. This work represents a first step towards the simultaneous multicomponent enantiospecific analysis of complex mixtures, a capability that will have substantial impact on metabolism studies, metabolic phenotyping, chemical reaction monitoring, and many other fields where complex mixtures containing chiral molecules require efficient characterisation.
KW - complex mixture
KW - mixture effect
KW - multicomponent
KW - NMR spectroscopy
KW - simultaneous enantiospecific analysis
UR - http://www.scopus.com/inward/record.url?scp=85093510068&partnerID=8YFLogxK
U2 - 10.1002/anie.202011727
DO - 10.1002/anie.202011727
M3 - Article
C2 - 32959941
AN - SCOPUS:85093510068
SN - 1433-7851
VL - 59
SP - 23615
EP - 23619
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 52
ER -