© 2015 Elsevier B.V. Simultaneous detection of Salmonella enterica, Listeria monocytogenes and Escherichia coli based on triple-tagging multiplex PCR and electrochemical magneto genosensing on silica magnetic particles is reported. A set of tagging primers were selected for the specific amplification of yfiR (375bp), hlyA (234bp) and eaeA (151bp), being one of the primers for each set labelled with fluorescein, biotin and digoxigenin coding for S. enterica, L. monocytogenes and E. coli, respectively. Afterwards, electrochemical magneto genosensing of the bacteria was achieved by using silica magnetic particles as a carrier and three different electrochemical reporters, specific for each pathogen. This method was able to clearly distinguish among the pathogenic bacteria tested within 50min, with detection limits ranging from 12 to 46pgμL-1.
|Journal||Biosensors and Bioelectronics|
|Publication status||Published - 5 Dec 2015|
- Electrochemical genosensing
- Foodborne bacteria
- Magneto-actuated electrode
- Simultaneous detection
- Triple-tagging multiplex PCR