Significance of tagI and mfd genes in the virulence of non-typeable Haemophilus influenzae

Denis A. Spricigo; Pilar Cortés; David Moranta; Jordi Barbé; José Antonio Bengoechea; Montserrat Llagostera

doi:10.2436/20.1501.01.218

Significance of tagI and mfd genes in the virulence of non-typeable Haemophilus influenzae

Translated title of the contribution: Significance of tagI and mfd genes in the virulence of non-typeable Haemophilus influenzae

Denis A. Spricigo, Pilar Cortés, David Moranta, Jordi Barbé, José Antonio Bengoechea, Montserrat Llagostera

Research output: Contribution to journal › Article › Research › peer-review

1 Citation (Scopus)

Abstract

© 2014, Sociedad Espanola de Microbiologia. All rights reserevd. Non-typeable Haemophilus infl uenzae (NTHi) is an opportunist pathogen well adapted to the human upper respiratory tract and responsible for many respiratory diseases. In the human airway, NTHi is exposed to pollutants, such as alkylating agents, that damage its DNA. In this study, we examined the significance of genes involved in the repair of DNA alkylation damage in NTHi virulence. Two knockout mutants, tagI and mfd, encoding N3methyladenine-DNA glycosylase I and the key protein involved in transcription-coupled repair, respectively, were constructed and their virulence in a BALB/c mice model was examined. This work shows that N3-methyladenine-DNA glycosylase I is constitutively expressed in NTHi and that it is relevant for its virulence.

Translated title of the contribution	Significance of tagI and mfd genes in the virulence of non-typeable Haemophilus influenzae
Original language	Multiple languages
Pages (from-to)	159-164
Journal	International Microbiology
Volume	17
Issue number	3
DOIs	https://doi.org/10.2436/20.1501.01.218
Publication status	Published - 1 Jan 2015

Keywords

Alkylating agents
Genes tagI and mfd
Haemophilus infl uenzae
Virulence

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Spricigo, D. A., Cortés, P., Moranta, D., Barbé, J., Bengoechea, J. A., & Llagostera, M. (2015). Significance of tagI and mfd genes in the virulence of non-typeable Haemophilus influenzae. International Microbiology, 17(3), 159-164. https://doi.org/10.2436/20.1501.01.218

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AU - Spricigo, Denis A.

AU - Cortés, Pilar

AU - Moranta, David

AU - Barbé, Jordi

AU - Bengoechea, José Antonio

AU - Llagostera, Montserrat

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AB - © 2014, Sociedad Espanola de Microbiologia. All rights reserevd. Non-typeable Haemophilus infl uenzae (NTHi) is an opportunist pathogen well adapted to the human upper respiratory tract and responsible for many respiratory diseases. In the human airway, NTHi is exposed to pollutants, such as alkylating agents, that damage its DNA. In this study, we examined the significance of genes involved in the repair of DNA alkylation damage in NTHi virulence. Two knockout mutants, tagI and mfd, encoding N3methyladenine-DNA glycosylase I and the key protein involved in transcription-coupled repair, respectively, were constructed and their virulence in a BALB/c mice model was examined. This work shows that N3-methyladenine-DNA glycosylase I is constitutively expressed in NTHi and that it is relevant for its virulence.

KW - Alkylating agents

KW - Genes tagI and mfd

KW - Haemophilus infl uenzae

KW - Virulence

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