Siglec-1 Is a Novel Dendritic Cell Receptor That Mediates HIV-1 Trans-Infection Through Recognition of Viral Membrane Gangliosides

Nuria Izquierdo-Useros, Maier Lorizate, Maria C. Puertas, Maria T. Rodriguez-Plata, Nadine Zangger, Elina Erikson, Maria Pino, Itziar Erkizia, Bärbel Glass, Bonaventura Clotet, Oliver T. Keppler, Amalio Telenti, Hans Georg Kräusslich, Javier Martinez-Picado

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Abstract

Dendritic cells (DCs) are essential antigen-presenting cells for the induction of immunity against pathogens. However, HIV-1 spread is strongly enhanced in clusters of DCs and CD4+ T cells. Uninfected DCs capture HIV-1 and mediate viral transfer to bystander CD4+ T cells through a process termed trans-infection. Initial studies identified the C-type lectin DC-SIGN as the HIV-1 binding factor on DCs, which interacts with the viral envelope glycoproteins. Upon DC maturation, however, DC-SIGN is down-regulated, while HIV-1 capture and trans-infection is strongly enhanced via a glycoprotein-independent capture pathway that recognizes sialyllactose-containing membrane gangliosides. Here we show that the sialic acid-binding Ig-like lectin 1 (Siglec-1, CD169), which is highly expressed on mature DCs, specifically binds HIV-1 and vesicles carrying sialyllactose. Furthermore, Siglec-1 is essential for trans-infection by mature DCs. These findings identify Siglec-1 as a key factor for HIV-1 spread via infectious DC/T-cell synapses, highlighting a novel mechanism that mediates HIV-1 dissemination in activated tissues. © 2012 Izquierdo-Useros et al.
Original languageEnglish
Article numbere1001448
JournalPLoS Biology
Volume10
Issue number12
DOIs
Publication statusPublished - 1 Dec 2012

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    Izquierdo-Useros, N., Lorizate, M., Puertas, M. C., Rodriguez-Plata, M. T., Zangger, N., Erikson, E., Pino, M., Erkizia, I., Glass, B., Clotet, B., Keppler, O. T., Telenti, A., Kräusslich, H. G., & Martinez-Picado, J. (2012). Siglec-1 Is a Novel Dendritic Cell Receptor That Mediates HIV-1 Trans-Infection Through Recognition of Viral Membrane Gangliosides. PLoS Biology, 10(12), [e1001448]. https://doi.org/10.1371/journal.pbio.1001448