Shu complex SWS1-SWSAP1 promotes early steps in mouse meiotic recombination

Carla M. Abreu, Rohit Prakash, Peter J. Romanienko, Ignasi Roig, Scott Keeney, Maria Jasin

Research output: Contribution to journalArticleResearch

9 Citations (Scopus)

Abstract

© 2018, The Author(s). The DNA-damage repair pathway homologous recombination (HR) requires factors that promote the activity of strand-exchange protein RAD51 and its meiosis-specific homolog DMC1. Here we show that the Shu complex SWS1-SWSAP1, a candidate for one such HR regulator, is dispensable for mouse viability but essential for male and female fertility, promoting the assembly of RAD51 and DMC1 on early meiotic HR intermediates. Only a fraction of mutant meiocytes progress to form crossovers, which are crucial for chromosome segregation, demonstrating crossover homeostasis. Remarkably, loss of the DNA damage checkpoint kinase CHK2 rescues fertility in females without rescuing crossover numbers. Concomitant loss of the BRCA2 C terminus aggravates the meiotic defects in Swsap1 mutant spermatocytes, suggesting an overlapping role with the Shu complex during meiotic HR. These results demonstrate an essential role for SWS1-SWSAP1 in meiotic progression and emphasize the complex interplay of factors that ensure recombinase function.
Original languageEnglish
Article number3961
JournalNature Communications
Volume9
DOIs
Publication statusPublished - 1 Dec 2018

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