Short communication: Intra- and inter-individual milk microbiota variability in healthy and infected water buffalo udder quarters

Carlotta Catozzi, Anna Cuscó, Cristina Lecchi, Andrea Talenti, Alessandra Martucciello, Giovanna Cappelli, Armand Sanchez Bonastre, Olga Francino, Fabrizio Ceciliani

Research output: Contribution to journalArticleResearch

Abstract

© 2019 American Dairy Science Association The concept that ruminant mammary gland quarters are anatomically and physiologically unrelated has been recently challenged by immunological evidence. How this interdependence reflects on individual quarter milk microbiota is unknown. The aim of the present study was to cover this gap by investigating the interdependence of quarters among the same mammary gland at the milk microbiota level using next-generation sequencing of the V4–16S rRNA gene. A total of 52 samples were included in this study and classified as healthy or affected by subclinical mastitis. Extraction of DNA, amplification of the V4–16S rRNA gene, and sequencing using Ion Torrent Personal Genome Machine (Thermo Fisher Scientific, Waltham, MA) were carried out. We found that the intra-individual variability was lower than the inter-individual one. The present findings further support at milk microbiota level the hypothesis of the interdependence of quarters, as previously demonstrated following immunological studies, suggesting that individual factors (e.g., immunity, genetics) may have a role in modulating milk microbiota.
Original languageEnglish
Pages (from-to)7476-7482
Number of pages7
JournalJournal of Dairy Science
Volume102
Issue number8
DOIs
Publication statusPublished - 1 Aug 2019

Keywords

  • milk microbiota
  • quarter interdependence
  • water buffalo
  • Mammary Glands, Animal/immunology
  • Mastitis, Bovine/immunology
  • Microbiota
  • Animals
  • Cattle
  • Buffaloes/genetics
  • Female
  • High-Throughput Nucleotide Sequencing
  • RNA, Ribosomal, 16S
  • Milk
  • INTERDEPENDENCE
  • EVOLUTION
  • MASTITIS

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