Sex identification of wolf (Canis lupus) using non-invasive samples

Natalia Sastre, Olga Francino, Gabriel Lampreave, Vladimir V. Bologov, José María López-Martín, Armand Sánchez, Oscar Ramírez

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

We have developed new specific primers for sex determination from forensic samples of wolves (Canis lupus), such as hair, saliva, faecal, tooth and urine samples. In order to improve molecular sexing, we performed a multiplex semi-nested polymerase chain reaction (PCR) and several replicated amplifications per sample to avoid errors in low quantity DNA samples, such as allelic dropout and false alleles. The sex of individuals is automatically determined by capillary electrophoresis with a fluorescently labelled internal sex-specific primer from each pair. Our method yielded sex identification on 100% of invasive samples and 93% of forensic samples, being one of the highest success rates obtained from wild animals. © 2008 Springer Science+Business Media B.V.
Original languageEnglish
Pages (from-to)555-558
JournalConservation Genetics
Volume10
DOIs
Publication statusPublished - 1 Jun 2009

Keywords

  • Canis lupus
  • Non-invasive samples
  • Sex identification
  • Sex-specific primers
  • Wolf

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