Acute myocardial infarction (AMI) is one of the major causes of mortality and morbidity worldwide. Despite the efforts being made, there is a lack of early markers for the prevention, diagnosis and treatment of ischemic syndromes. Proteomic expression profiling technologies are a highly important tool for research into new serum biomarkers for the diagnosis and prognosis of acute coronary syndromes. Methods: Serum samples were sub-fractionated with different methods for the depletion of high-abundance proteins. The low-abundance fraction was analyzed by two-dimensional electrophoresis (2-DE), followed by protein identification with mass-spectrometry (MALDI-TOF). The proteomic profiles of serum samples from AMI patients and controls were analyzed and compared. Results: Through depletion of six high-abundance proteins in 2-DE analysis of serum samples, 569 spots were detected, of which 131 spots were only detected in the AMI group and 27 were only detected in controls. The comparative analysis between AMI-patients and controls revealed a group of differential protein spots involved in seven different biological functions. The main changes were found in proteins involved in the immune system and lipid metabolism. Conclusions: In this study, by using a 2-DE differential approach, we developed a highly reproducible methodology for the analysis of coordinated changes in serum proteome patterns that occur within the first 6 hours after the onset of an AMI. © 2010 Elsevier España, S.L. and SEA.
|Journal||Clinica e Investigacion en Arteriosclerosis|
|Publication status||Published - 1 Jul 2011|