TY - JOUR
T1 - Serologic and virologic investigations into pestivirus infection in wild and domestic ruminants in the Pyrenees (NE Spain)
AU - Marco, I.
AU - Rosell, R.
AU - Cabezón, O.
AU - Beneria, M.
AU - Mentaberre, G.
AU - Casas, E.
AU - Hurtado, A.
AU - López-Olvera, J. R.
AU - Lavín, S.
PY - 2009/8/1
Y1 - 2009/8/1
N2 - An outbreak of disease associated to a border disease virus was described in the Southern chamois (Rupicapra pyrenaica) in Spain in 2002. Sera and/or spleen samples from 57 mouflon, 15 red deer, 21 roe deer, 3 fallow deer, 55 sheep, 32 cattle, and 68 goats sharing the chamois habitat were studied. An antibody ELISA test yielded an inconclusive result in 2 mouflon and positive results in 5 goat sera. Comparative virus neutralization tests were performed on the 2 inconclusive mouflons, 3 of the 5 seropositive goats, 55 sheep and 32 cattle, using 6 pestivirus strains. Positive results were obtained in 1 mouflon, 2 goats, 69% of sheep and 78% of cattle. Virological investigations performed with an antigen ELISA test yielded negative results in 21 goats and 39 mouflons, the result in 1 mouflon being inconclusive. PCR performed on 12 goats and the inconclusive mouflon gave negative results. These results suggested that it is unlikely that chamois BDV is infecting wild and domestic ruminants. © 2008 Elsevier Ltd. All rights reserved.
AB - An outbreak of disease associated to a border disease virus was described in the Southern chamois (Rupicapra pyrenaica) in Spain in 2002. Sera and/or spleen samples from 57 mouflon, 15 red deer, 21 roe deer, 3 fallow deer, 55 sheep, 32 cattle, and 68 goats sharing the chamois habitat were studied. An antibody ELISA test yielded an inconclusive result in 2 mouflon and positive results in 5 goat sera. Comparative virus neutralization tests were performed on the 2 inconclusive mouflons, 3 of the 5 seropositive goats, 55 sheep and 32 cattle, using 6 pestivirus strains. Positive results were obtained in 1 mouflon, 2 goats, 69% of sheep and 78% of cattle. Virological investigations performed with an antigen ELISA test yielded negative results in 21 goats and 39 mouflons, the result in 1 mouflon being inconclusive. PCR performed on 12 goats and the inconclusive mouflon gave negative results. These results suggested that it is unlikely that chamois BDV is infecting wild and domestic ruminants. © 2008 Elsevier Ltd. All rights reserved.
KW - Cattle
KW - Goat
KW - Pestivirus
KW - Sheep
KW - Wild ungulates
U2 - https://doi.org/10.1016/j.rvsc.2008.10.014
DO - https://doi.org/10.1016/j.rvsc.2008.10.014
M3 - Article
SN - 0034-5288
VL - 87
SP - 149
EP - 153
JO - Research in Veterinary Science
JF - Research in Veterinary Science
ER -