Seroepidemiological study of Toxoplasma gondii in equids in different European countries

David Cano-Terriza, Juan J. Franco, Eduard Jose-Cunilleras, Francesco Buono, Sonia Almería, Vincenzo Veneziano, Eduardo Alguacil, Jesús García, Isabelle Villena, Jitender P. Dubey, Débora Jiménez-Martín*, Ignacio García-Bocanegra

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Toxoplasmosis, caused by the obligate intracellular protozoan Toxoplasma gondii, is a worldwide parasitic zoonosis. A cross-sectional study was carried out to determine the exposure to T. gondii in equids in Europe. Serum samples from 1399 equids (1085 horses, 238 donkeys, and 76 mules/hinnies) bred in four European countries (Italy, Spain, United Kingdom [UK], and Ireland) were collected during the period of 2013–2021. The overall seroprevalence of T. gondii was 18.9% (95% Confidence Interval [CI]: 16.9–21.0) by using the modified agglutination test (MAT) at a cut-off of 1:25. Seropositivity by country was 27.1% in Italy, 16.6% in Spain, 12.0% in UK and 7.0% in Ireland. Anti-T. gondii antibodies were detected in 12.8% of the horses, 43.7% of the donkeys, and in 28.9% of the mules/hinnies. A Generalized Estimating Equation (GEE) analysis was carried out to study the associations between seropositivity and explanatory variables related to individuals, herds, and management measures on these herds, selected based on the bivariate analysis. The risk for being seropositive for T. gondii was 5.3 and 2.7 times higher in donkeys and mules/hinnies than in horses, respectively. In addition, significantly higher seropositivity was observed in horses from herds that used disinfectants less than once a week (13.9%; p = 0.038, odds ratio [OR] = 1.6; 95% CI: 1.03–2.62) compared with those from herds that performed weekly disinfection of the facilities (9.4%). This is the first large-scale seroepidemiological study on T. gondii comprising horses, donkeys, and mules/hinnies in Europe and the first report of T. gondii exposure in horses from Ireland and UK. We found a widespread distribution of T. gondii among equid populations in different European countries. The seroprevalence found in these species, especially in donkeys and mules/hinnies, highlights the potential risk of human infection through the consumption of their raw/undercooked milk or meat.

Original languageEnglish
Pages (from-to)276-283
Number of pages8
JournalZoonoses and Public Health
Volume70
Issue number3
DOIs
Publication statusPublished - 23 Jan 2023

Keywords

  • Animals
  • Antibodies, Protozoan
  • Cross-Sectional Studies
  • Equidae/parasitology
  • Europe/epidemiology
  • Horse Diseases/epidemiology
  • Horses
  • Humans
  • Risk Factors
  • Seroepidemiologic Studies
  • Toxoplasma
  • Toxoplasmosis, Animal/epidemiology

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