Sequential G-banding FISH on human sperm chromosomes

P. Colls, C. Templado, O. Martínez-Pasarell, F. Darroudi, A. T. Natarajan

    Research output: Contribution to journalArticleResearchpeer-review

    5 Citations (Scopus)

    Abstract

    A method that allows the performance of double-colour chromosome painting (FISH) on previously G-banded human sperm metaphases has been developed. Sperm chromosomes were obtained by using the fusion technique between zona-free hamster oocytes and human spermatozoa. Single- and double-colour chromosome painting was performed using DNA libraries specific for chromosomes X, Y and 21 on either unstained or G-banded preparations. The hybridization efficiency was very high (98%). The sequential staining technique is very useful for analyses of structural (stable) and numerical chromosome aberrations in human sperm and thus can increase the efficiency of the human sperm-hamster oocytes fusion system to assess the risk to human germ cells as a result of endogenous and exogenous factors.
    Original languageEnglish
    Pages (from-to)457-461
    JournalChromosome Research
    Volume5
    DOIs
    Publication statusPublished - 1 Jan 1997

    Keywords

    • Double-colour FISH
    • G-banding
    • Human sperm chromosomes

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