Selenite Downregulates STAT3 Expression and Provokes Lymphocytosis in the Liver of Chronically Exposed Syrian Golden Hamsters

María E. Camacho-Moll, Adriana Sampayo-Reyes, Fabiola Castorena-Torres, Gerardo Lozano-Garza, Gabriela Alarcón-Galván, Alba Hernández, Ricard Marcos, Juan Alcocer-González, Reyes Tamez-Guerra, Mario Bermúdez de León

Research output: Contribution to journalArticleResearchpeer-review

1 Downloads (Pure)

Abstract

Arsenic is considered a worldwide pollutant that can be present in drinking water. Arsenic exposure is associated with various diseases, including cancer. Antioxidants as selenite and α-tocopherol-succinate have been shown to modulate arsenic toxic effects. Since changes in STAT3 and PSMD10 gene expression have been associated with carcinogenesis, the aim of this study was to evaluate the effect of arsenic exposure and co-treatments with selenite or α-tocopherol-succinate on the expression of these genes, in the livers of chronically exposed Syrian golden hamsters. Animals were divided into six groups: (i) control, (ii) chronically treated with 100 ppm arsenic, (iii) treated with 6 ppm α-tocopherol-succinate (α-TOS), (iv) treated with 8.5 ppm selenite, (v) treated with arsenic + α-TOS, and (vi) treated with arsenic + selenite. Urine samples and livers were collected after 20 weeks of continuous exposure. The urine samples were analyzed for arsenic species by atomic absorption spectrophotometry, and real-time RT-qPCR analysis was performed for gene expression evaluation. A reduction in STAT3 expression was observed in the selenite-treated group. No differences in PSMD10 expression were found among groups. Histopathological analysis revealed hepatic lymphocytosis in selenite-treated animals. As a conclusion, long-term exposure to arsenic does not significantly alter the expression of STAT3 and PSMD10 oncogenes in the livers of hamsters; however, selenite down-regulates STAT3 expression and provokes lymphocytosis.

Original languageEnglish
Article number5614
Pages (from-to)26: 5614
Number of pages12
JournalMolecules
Volume26
Issue number18
Publication statusPublished - 16 Sep 2021

Keywords

  • Administration, Oral
  • Animals
  • Antioxidants/administration & dosage
  • Arsenic
  • Arsenic/administration & dosage
  • Down-Regulation/drug effects
  • Hamster
  • Kaplan-Meier Estimate
  • Liver
  • Liver/drug effects
  • Lymphocytosis/chemically induced
  • Male
  • Mesocricetus
  • Oncogenes
  • Proteasome Endopeptidase Complex/genetics
  • STAT3 Transcription Factor/genetics
  • Selenious Acid/administration & dosage
  • Selenite
  • Weight Gain/drug effects
  • alpha-Tocopherol/pharmacology
  • α-tocopherol

Fingerprint

Dive into the research topics of 'Selenite Downregulates STAT3 Expression and Provokes Lymphocytosis in the Liver of Chronically Exposed Syrian Golden Hamsters'. Together they form a unique fingerprint.

Cite this