Selective neuroprotective effects of the S18Y polymorphic variant of UCH-L1 in the dopaminergic system

Maria Xilouri, Elli Kyratzi, Pothitos M. Pitychoutis, Zoi Papadopoulou-Daifoti, Celine Perier, Miquel Vila, Matina Maniati, Ayse Ulusoy, Deniz Kirik, David S. Park, Keiji Wada, Leonidas Stefanis

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24 Citations (Scopus)

Abstract

Genetic studies have implicated the neuronal ubiquitin C-terminal hydrolase (UCH) protein UCH-L1 in Parkinson's disease (PD) pathogenesis. Moreover, the function of UCH-L1 may be lost in the brains of PD and Alzheimer's disease patients. We have previously reported that the UCH-L1 polymorphic variant S18Y, potentially protective against PD in population studies, demonstrates specific antioxidant functions in cell culture. Albeit genetic, biochemical and neuropathological data support an association between UCH-L1, PD, synaptic degeneration and oxidative stress, the relationship between the dopaminergic system and UCH-L1 status remains obscure. In the current study, we have examined the dopaminergic system of mice lacking endogenous UCH-L1 protein (gracile axonal dystrophy mice). Our findings show that the lack of wild-type (WT) UCH-L1 does not influence to any significant degree the dopaminergic system at baseline or following injections of the neurotoxin methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Furthermore, using a novel intrastriatal adenoviral injection protocol, we have found that mouse nigral neurons retrogradely transduced with S18Y UCH-L1, but not the WT protein, are significantly protected against MPTP toxicity. Overall, these data provide evidence for an antioxidant and neuroprotective effect of the S18Y variant of UCH-L1, but not of the WT protein, in the dopaminergic system, and may have implications for the pathogenesis of PD or related neurodegenerative conditions, in which oxidative stress might play a role. © The Author 2011. Published by Oxford University Press. All rights reserved.
Original languageEnglish
Article numberddr521
Pages (from-to)874-889
JournalHuman Molecular Genetics
Volume21
Issue number4
DOIs
Publication statusPublished - 1 Feb 2012

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