Root cell patterning: A primary target for aluminium toxicity in maize

Snezhanka Doncheva, Montserrat Amenós, Charlotte Poschenrieder, Juan Barceló

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Abstract

The short-term influence (5-180 min) of 50 μM Al on cell division was investigated in root tips of two Zea mays L. varieties differing in Al-resistance. The incorporation of bromodeoxyuridine into S-phase nuclei was visualized by immunofluorescence staining using confocal laser fluorescence microscopy. In Al-sensitive plants 5 min Al exposure was enough to inhibit cell division in the proximal meristem (250-800 μm from the tip). After 10 or 30 min with Al only, a few S-phase nuclei were found in the cortical initials. By contrast, cell division was stimulated in the distal elongation zone (2.5-3.1 mm). After 180 min the protrusion of an incipient lateral root was observed in this zone. These observations suggest a fast change in cell patterning rather than a general cariotoxic effect after exposure to Al for a short time. No such changes were found in Al-resistant maize. This is the first report showing such fast Al-induced alterations in the number and the position of dividing cells in root tips. The observation that similar changes were induced by a local supply of naphthylphthalamic acid to the distal transition zone suggests that inhibition of auxin transport plays a role in the Al-induced alteration of root cell patterning. © The Author [2005]. Published by Oxford University Press. All rights reserved.
Original languageEnglish
Pages (from-to)1213-1220
JournalJournal of Experimental Botany
Volume56
DOIs
Publication statusPublished - 1 Apr 2005

Keywords

  • Aluminium toxicity
  • Auxin
  • Cell division
  • Naphthylphthalamic acid
  • Root development
  • Zea mays L.

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    Doncheva, S., Amenós, M., Poschenrieder, C., & Barceló, J. (2005). Root cell patterning: A primary target for aluminium toxicity in maize. Journal of Experimental Botany, 56, 1213-1220. https://doi.org/10.1093/jxb/eri115