Role of UEV-1A, a homologue of the tumor suppressor protein TSG101, in protection from DNA damage

Timothy M. Thomson, Hajji Khalid, Juan José Lozano, Elena Sancho, Joaquín Ariño

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

The open reading frame YGL087c in the budding yeast Saccharomyces cerevisiae genome encodes a polypeptide highly similar to the human UEV (ubiquitin-conjugating E2 enzyme variant) proteins, which have been proposed to belong to a family of putative dominant negative ubiquitin regulators. Deletion of the YGL087c open reading frame yields viable cells which are sensitive to UV irradiation or methyl methanesulfonate, but not to hydroxyurea. This phenotype is reminiscent of that of rad mutants and suggests that the YGL087c-encoded protein functions in a process related to tolerance to DNA damage. We also show that the mutant phenotype is fully complemented by expression of the human UEV-1A cDNA and we propose that UEV-1 proteins could also have a role in protecting higher eukaryotic cells from DNA damaging agents.
Original languageEnglish
Pages (from-to)49-52
JournalFEBS Letters
Volume423
DOIs
Publication statusPublished - 13 Feb 1998

Keywords

  • DNA repair
  • Saccharomyces cerevisiae
  • Ubiquitination

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