Role of the endocannabinoid system in the emotional manifestations of osteoarthritis pain

Carmen La Porta, S. Andreea Bura, Jone Llorente-Onaindia, Antoni Pastor, Francisco Navarrete, María Salud García-Gutierrez, Rafael De La Torre, Jorge Manzanares, Jordi Monfort, Rafael Maldonado

Research output: Contribution to journalArticleResearchpeer-review

50 Citations (Scopus)


© 2015 International Association for the Study of Pain. In this study, we investigated the role of the endocannabinoid system (ECS) in the emotional and cognitive alterations associated with osteoarthritis pain. The monosodium iodoacetate model was used to evaluate the affective and cognitive manifestations of osteoarthritis pain in type 1 (CB1R) and type 2 (CB2R) cannabinoid receptor knockout and wild-type mice and the ability of CB1R (ACEA) and CB2R (JWH133) selective agonists to improve these manifestations during a 3-week time period. The levels of the endocannabinoids anandamide (AEA) and 2-Arachidonoylglycerol (2-AG) were measured in plasma and brain areas involved in the control of these manifestations. Patients with knee osteoarthritis and healthy controls were recruited to evaluate pain, affective, and cognitive symptoms, as well as plasma endocannabinoid levels and cannabinoid receptor gene expression in peripheral blood lymphocytes. The affective manifestations of osteoarthritis were enhanced in CB1R knockout mice and absent in CB2R knockouts. Interestingly, both ACEA and JWH133 ameliorated the nociceptive and affective alterations, whereas ACEA also improved the associated memory impairment. An increase of 2-AG levels in prefrontal cortex and plasma was observed in this mouse model of osteoarthritis. In agreement, an increase of 2-AG plasmatic levels and an upregulation of CB1R and CB2R gene expression in peripheral blood lymphocytes were observed in patients with osteoarthritis compared with healthy subjects. Changes found in these biomarkers of the ECS correlated with pain, affective, and cognitive symptoms in these patients. The ECS plays a crucial role in osteoarthritis and represents an interesting pharmacological target and biomarker of this disease.
Original languageEnglish
Pages (from-to)2001-2012
Issue number10
Publication statusPublished - 1 Oct 2015


  • 2-Arachidonoylglycerol
  • Anandamide
  • Animal model
  • Anxiety
  • Cannabinoid receptor 1
  • Cannabinoid receptor 2


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