RGD-based cell ligands for cell-targeted drug delivery act as potent trophic factors

Joan Domingo-Espín, Valérie Petegnief, Núria de Vera, Oscar Conchillo-Solé, Paolo Saccardo, Ugutz Unzueta, Esther Vazquez, Juan Cedano, Luciana Negro, Xavier Daura, Hugo Peluffo, Anna M. Planas, Antonio Villaverde, Neus Ferrer-Miralles

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

Integrin-binding, Arg-Gly-Asp (RGD)-containing peptides are the most widely used agents to deliver drugs, nanoparticles, and imaging agents. Although in nature, several protein-mediated signal transduction events depend on RGD motifs, the potential of RGD-empowered materials in triggering undesired cell-signaling cascades has been neglected. Using an RGD-functionalized protein nanoparticle, we show here that the RGD motif acts as a powerful trophic factor, supporting extracellular signal-regulated kinase 1/2 (ERK1/2)-linked cell proliferation and partial differentiation of PC12 cells, a neuronlike model. From the Clinical Editor: This work focuses on RGD peptides, which are among the most commonly used tags for targeted drug delivery. They also promote protoneurite formation and expression of neuronal markers (MAP2) in model PC12 cells, which is an unexpected but relevant event in the functionalization of drugs and their nanocarriers. © 2012 Elsevier Inc.
Original languageEnglish
Pages (from-to)1263-1266
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume8
DOIs
Publication statusPublished - 1 Nov 2012

Keywords

  • Biomaterials
  • Integrins
  • Neurons
  • Protein nanoparticles
  • RGD

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