During the Last Glacial cycle (MIS 4 to MIS 2), the isthmic Pyrenees mountain range acted as a biogeographical barrier, effectively restricting faunal exchanges to its western and eastern terminations. The study of the composition of megaherbivore communities has revealed the transitional character of the Cantabrian region (northwest Iberian Peninsula) during the MIS 3, between western European cold faunas and southern Iberian temperate faunas. In the Catalan area (northeastern Iberian Peninsula), cold-adapted faunas are frequently recorded during the cold pulses of MIS 3 and MIS 2, albeit to a lesser extent. In the present paper, we provide the first direct evidence of a muskox record in the Iberian Peninsula, together with a morphometric study and the results of collagen δ13C and δ15N analysis. The results reveal that the Arbreda specimen fits biometrically within the Late Holocene Umingmak specimens (Banks Island, Canada). Moreover, its isotopic values fall into the range of Late Pleistocene Mammoth steppe muskoxen, which indicates a typical ecological behaviour for muskox. Indeed, it suggests a niche partitioning of this cold-adapted specimen in relation to other Gravettian Iberian fauna. Chronologically, this occurrence is interpreted as an isolated southward expansion of the biogeographical limits of muskox during the Greenland Stadial 4, in the latest MIS 3.