Retrospective detection of Porcine circovirus 3 (PCV-3) in pig serum samples from Spain

F. Klaumann, G. Franzo, M. Sohrmann, F. Correa-Fiz, M. Drigo, J. I. Núñez, M. Sibila, J. Segalés

Research output: Contribution to journalArticleResearch

35 Citations (Scopus)

Abstract

© 2018 The Authors. Transboundary and Emerging Diseases Published by Blackwell Verlag GmbH Porcine circovirus 3 (PCV-3) is an emerging circovirus species that has recently been reported in different countries around the world, suggesting a widespread circulation. In this study, sera samples originating from 654 pigs of different production phases and clinical/pathological conditions, submitted for diagnostic purposes between 1996 and 2017, were randomly selected. Detection of PCV-3 genome in such samples was attempted with a previously described PCR method, and the partial genome sequence was obtained from selected PCV-3-positive samples from different years. Compiled data confirmed that PCV-3 has been circulating in the Spanish pig population since 1996. The overall frequency of PCV-3 PCR-positive samples in the study period was 11.47% (75 of 654). Phylogenetic analysis of twelve PCV-3 partial sequences obtained showed a high nucleotide identity with the already known PCV-3 sequences, with minor variations among years. No significant correlation was found between the detection of PCV-3 and any production phase nor clinical/pathological condition. These results confirm PCV-3 circulation at least since 1996 in the Spanish pig population with a low/moderate frequency. Although the information obtained was limited, PCV-3 did not appear to be linked to any specific pathological condition or age group.
Original languageEnglish
Pages (from-to)1290-1296
JournalTransboundary and Emerging Diseases
Volume65
DOIs
Publication statusPublished - 1 Oct 2018

Keywords

  • polymerase chain reaction
  • Porcine circovirus 3
  • retrospective
  • sequencing
  • Spain

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