Retinoic acid-induced differentiation into astrocytes and glutamatergic neurons is associated with expression of functional and activable phospholipase D

María A. Rujano, Pilar Pina, Joan M. Servitja, Ana M. Ahumada, Fernando Picatoste, Jaume Farrés, Josefa Sabrià

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4 Citations (Scopus)

Abstract

Phospholipase D (PLD) activity in mammalian cells has been associated with cell proliferation and differentiation. Here, we investigated the expression of PLD during differentiation of pluripotent embryonal carcinoma cells (P19) into astrocytes and neurons. Retinoic acid (RA)-induced differentiation increased PLD1 and PLD2 mRNA levels and PLD activity that was responsive to phorbol myristate acetate. Various agonists of membrane receptors activated PLD in RA-differentiated cells. Glutamate was a potent activator of PLD in neurons but not in astrocytes, whereas noradrenaline and carbachol increased PLD activity only in astrocytes. P19 neurons but not astrocytes released glutamate in response to a depolarizing stimulus, confirming the glutamatergic phenotype of these neurons. These results indicate upregulation of PLD gene expression associated with RA-induced neural differentiation. © 2004 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)387-392
JournalBiochemical and Biophysical Research Communications
Volume316
DOIs
Publication statusPublished - 2 Apr 2004

Keywords

  • Astrocyte
  • Carbachol
  • Cellular differentiation
  • Glutamate
  • Neuron
  • Noradrenaline
  • P19 cells
  • Phospholipase D
  • Retinoic acid

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    Rujano, M. A., Pina, P., Servitja, J. M., Ahumada, A. M., Picatoste, F., Farrés, J., & Sabrià, J. (2004). Retinoic acid-induced differentiation into astrocytes and glutamatergic neurons is associated with expression of functional and activable phospholipase D. Biochemical and Biophysical Research Communications, 316, 387-392. https://doi.org/10.1016/j.bbrc.2004.02.057