Resveratrol supplementation during in vitro maturation improves embryo development of prepubertal goat oocytes selected by brilliant cresyl blue staining

Anna Rita Piras, Irene Menéndez-Blanco, Sandra Soto-Heras, Maria Gracia Catalá, Dolors Izquierdo, Luisa Bogliolo, Maria Teresa Paramio*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

This study aimed to investigate the effect of resveratrol supplementation in maturation medium on the developmental ability and bioenergetic\oxidative status of prepubertal goat oocytes selected by brilliant cresyl blue (BCB). Oocytes collected from slaughterhouse-derived ovaries were selected by 13 μM BCB staining and classified as grown BCB+ and growing BCBoocytes. All oocytes were matured in vitro in our conventional maturation medium and supplemented with 1 μM (BCB+R and BCB-R) and without (Control groups: BCB+C and BCB-C) resveratrol. After 24 h, IVM-oocytes were fertilized with fresh semen and presumptive zygotes were in vitro cultured for 8 days. Oocytes were assessed for blastocyst development and quality, mitochondrial activity and distribution, and levels of GSH, ROS, and ATP. BCB+R (28.3%) oocytes matured with resveratrol presented significantly higher blastocyst development than BCB+C (13.0%) and BCB- groups (BCB-R: 8.3% and BCB-C: 4.7%). Resveratrol improved blastocyst development of BCB-R oocytes at the same rate as BCB+C oocytes. No differences were observed in blastocyst quality among groups. GSH levels were significantly higher in resveratrol groups (BCB+R: 36554.6; BCB-R: 34946.7 pixels/oocyte) than in control groups (BCB+C: 27624.0; BCB-C: 27655.4 pixels/ oocyte). No differences were found in mitochondrial activity, ROS level, and ATP content among the groups. Resveratroltreated oocytes had a higher proportion of clustered active mitochondria in both BCB groups (BCB+R: 73.07%; BCB-R: 79.16%) than control groups (BCB+C: 19.35%; BCB-C: 40%). In conclusion, resveratrol increased blastocyst production from oocytes of prepubertal goats, particularly in better quality oocytes (BCB+).
Original languageEnglish
Pages (from-to)113-120
Number of pages8
JournalJournal of Reproduction and Development
Volume65
Issue number2
DOIs
Publication statusPublished - 12 Apr 2019

Keywords

  • ADENOSINE-TRIPHOSPHATE
  • Animals
  • Antioxidant
  • BOVINE OOCYTES
  • Blastocyst
  • Blastocyst/drug effects
  • COMPETENCE
  • Cell Separation/methods
  • Cells, Cultured
  • Coloring Agents/chemistry
  • EXPRESSION
  • Embryo, Mammalian
  • Embryonic Development/drug effects
  • FERTILIZATION
  • Female
  • Fertilization in Vitro/veterinary
  • GLUTATHIONE
  • Goats
  • In Vitro Oocyte Maturation Techniques/methods
  • In vitro fertilization (IVF)
  • Juvenile ruminants
  • MATURE OOCYTES
  • MITOCHONDRIAL DISTRIBUTION
  • Male
  • Oocytes/cytology
  • Oogenesis/drug effects
  • Oxazines/chemistry
  • PORCINE OOCYTES
  • PROMOTING FACTOR ACTIVITY
  • Resveratrol/pharmacology
  • Sexual Maturation/physiology
  • Staining and Labeling/methods

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