Response of human chondrocytes to a non-uniform distribution of hydrophilic domains on poly (ethyl acrylate-co-hydroxyethyl methacrylate) copolymers

Marcos Pérez Olmedilla, Natalia Garcia-Giralt, Manuel Monleón Pradas, Pere Benito Ruiz, José Luis Gómez Ribelles, Enric Cáceres Palou, Joan Carles Monllau García

    Research output: Contribution to journalArticleResearchpeer-review

    60 Citations (Scopus)

    Abstract

    A series of polymer and copolymer networks with varying hydrophilicity and distribution of the hydrophilic groups was synthesized and biologically tested with monolayer culture of human chondrocytes in vitro. Cell viability (MTT), proliferation (BrdU incorporation) and aggrecan expression (PG ELISA) were quantified at 7 and 14 days from seeding. Both assays (MTT and BrdU) showed complementary results that are consistent with positive cellular adhesion on the material. When human chondrocytes were cultured on polymer substrates in which the hydrophilic groups were homogeneously distributed, hydrophobic substrates showed higher values in all the biological parameters analysed. Adhesion, proliferation and viability decreased monotonously with the content of hydrophilic groups in the polymer chain. However poly(ethyl acrylate-co- hydroxyethyl methacrylate) copolymers, in which hydrophilic and hydrophobic nano-domains alternate as phase-separated domains, showed better results than the corresponding homopolymers. © 2005 Elsevier Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)1003-1012
    JournalBiomaterials
    Volume27
    Issue number7
    DOIs
    Publication statusPublished - 1 Mar 2006

    Keywords

    • Monolayer cell culture
    • PolyEA
    • PolyEMA
    • PolyHEA
    • PolyHEMA
    • Polymer network

    Fingerprint Dive into the research topics of 'Response of human chondrocytes to a non-uniform distribution of hydrophilic domains on poly (ethyl acrylate-co-hydroxyethyl methacrylate) copolymers'. Together they form a unique fingerprint.

    Cite this