Resolution of amino acid mixtures by an array of potentiometric sensors based on boronic acid derivative in a SIA flow system

M. Jańczyk, A. Kutyła-Olesiuk, X. Cetó, M. Del Valle, P. Ciosek, W. Wróblewski

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

The objective of this work was to perform the quantitative analysis of 4 amino acids mixtures: phenylalanine, tyrosine, ornithine and glutamic acid. For this purpose a potentiometric sensor array (electronic tongue) and Sequential Injection Analysis (SIA) measurement system were employed. The flow-through sensor array was composed of 6 miniaturized classical ion-selective electrodes based on polymeric membranes (plasticized PVC) containing 4- octyloxyphenylboronic acid as an ionophore and/or an ion exchanger. Partial Least Squares (PLS) analysis of the steady-state sensor array responses, measured in amino acids mixtures prepared by the SIA system (in two buffer solutions), permitted a correct quantitative analysis only of glutamic acid and ornithine. While a further chemometric treatment, involving the extraction of dynamic components of the transient response employing the Wavelet transform, the removal of less-significant coefficients by means of Causal Index pruning and training of an Artificial Neural Network (ANN) with the selected coefficients, allowed the simultaneous determination of the four amino acids. © 2013 Elsevier B.V.
Original languageEnglish
Pages (from-to)179-186
JournalSensors and Actuators, B: Chemical
Volume189
DOIs
Publication statusPublished - 18 Mar 2013

Keywords

  • Amino acids analysis
  • Automated electronic tongue
  • Boronic acids
  • Ion-selective electrodes
  • Sequential injection analysis

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