Resistance to osmotic stress of horse spermatozoa: The role of ionic pumps and their relationship to cryopreservation success

F. I. Caiza De La Cueva, M. R. Pujol, T. Rigau, S. Bonet, J. Miró, M. Briz, J. E. Rodriguez-Gill

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    28 Citations (Scopus)

    Abstract

    To study the resistance of horse spermatozoa against hyperosmotic stress, cells were incubated in solutions of 600 to 4000 mOsm(undisturbed media). Then, semen was immediately placed into an iso-osmotic solution (disrupted media). Incubation in undisturbed media decreased sperm viability in an osmolarity- and temperature-dependent manner. Viability was further decreased in disrupted media, with the effect dependent upon the initial osmolarity of the media and on the temperature. Treatment with ouabain or amiloride impaired the resistance of horse spermatozoa to hyperosmotic stress. Very few correlations were strong between viability after hyperosomotic stress and quality parameters of fresh and frozen-thawed horse semen. The results indicate that the usefulness of resistance to hyperosmotic stress in assessing frozen-thawed semen quality is compromised, since other factors are involved in the resistance to freezing-thawing. Both Na+/K+ ATP-ase and the Na+/H+ antiporter act in the resistance to hyperosmotic stress in horse spermatozoa.
    Original languageEnglish
    Pages (from-to)947-968
    JournalTheriogenology
    Volume48
    Issue number6
    DOIs
    Publication statusPublished - 15 Oct 1997

    Keywords

    • Freezing-thawing
    • Horse spermatozoa
    • Osmotic stress

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